Overview

Membrane proteins, blood plasma proteins, vaccines, and Ig fusions are just some of the classes of proteins which have proven very difficult to express in quantities large enough to feed the pipeline of therapeutic proteins. This conference will present strategies for improving the expression of these "finicky" proteins, as well as providing researchers with opportunities to network with groups which are successfully overcoming these obstacles.

Scientific Advisory Board
Dr. Deb Chatterjee, Associate Director, Senior Principal Scientist, Protein Expression Lab, SAIC-National Cancer Institute Frederick
Dr. Ajith Kamath, Associate Director, Protein Science, Pfizer Inc.
Dr. Karen Sitney, Director, Protein Science, Amgen Inc.
Dr. Geoffrey Waldo, Structural Biology, Los Alamos National Laboratory

 

Expression, Purification and Characterization Strategies

POET: Simultaneous Screening of Hundreds of Genes for Expression Success
Dr. James Hartley, Head, Protein Expression Laboratory, NCI-SAIC 
Production of Human Therapeutic Glycoproteins in Engineered Yeast
Dr. Natarajan Sethuraman, Associate Director, Product Development, GlycoFi, Inc. 
The LEX System™ - A Plant Based Expression System for Difficult to Express Proteins
Dr. John Gasdaska, Director, Bioanalytical Development, Biolex, Inc.
Manufacturing of High-Yield Soluble Protein Expression in E. coli by Screening of DNA Libraries
Prof. Pär Nordlund, Professor, Biochemistry and Biophysics, Stockholm University 
The Rapid, Inexpensive Chemical Synthesis of Proteins
Dr. James Burton, CEO, Psyche Pharmaceuticals, Inc. 
A Novel Human Expression System for Fully Human Glycosylation and Optimized Sialylation of Proteins
Dr. Steffen Goletz, GLYCOTOPE GmbH

Membrane Protein Initiative: Current Status
Dr. Peter Preusch, NIGMS, National Institutes of Health-Invited
Virtual Optimization for Protein Crystallization of Membrane Proteins
Dr. Larry DeLucas, Director, Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham 
Large-Scale Expression and Automated Purification of a G-Protein-Coupled Receptor for Structure Determination
Dr. Reinhard Grisshammer, Laboratory of Molecular Biology, NIDDK, National Institutes of Health
Automated On-Column Protein Purification Refolding: Selected Case Studies for Soluble and Transmembrane Proteins
Dr. Wes Swietnicki, Scientist, Toxinology U.S. Army Medical Research Institute of Infectious Diseases

 

Case Studies

A Pharmaceutical Working Paradigm for Difficult to Express Proteins
Dr. Felipe Monteclaro, Group Leader, Protein Chemistry, Schering AG Enabling Technologies
Change the Gene if You Can, the Protein if You Must
Dr. Dan Allison, Director, Production Development, ICOS Corporation
In Vitro Folding of beta1,4-galactosyltransferases
Dr. Boopathy Ramakrishnan, Senior Scientist, SAIC, NIH NCI
Expression and Purification of Tristetraprolin: An Arthritis-Suppressor Protein
Dr. Heping Cao, Research Fellow, Laboratory of Neurobiology, NIEHS/NIH/DHHS
Problems of Difficult to Express Proteins and Solutions 
Dr. Tauseef R. Butt, Research and Development, LifeSensors Inc.

Call for Sponsors and Exhibitors
Showcase your company’s expertise, brand your solutions and develop revenue opportunities with qualified decision-makers by becoming an Exhibitor or Sponsor of PEGS: The Protein Engineering Summit. We have a number of opportunities available, including 30-minute technology workshop presentations. To discuss sponsoring or exhibiting at this conference, please contact Carol Dinerstein at 781-972-5471 or dinerstein@healthtech.com.