2:30-5:00 Pre-conference Short Course Tutorial
Establishing Good Laboratory Practice for Gene Expression: A Practical Guide for the Laboratory
Dr. Andrew I. Brooks, Associate Professor, Environmental Medicine and Genetics; Director, Bionomics Research and Technology Center, University of Medicine and Dentistry of New Jersey and Associate Director, Technology Development - Rutgers University Cell and DNA Repository, Department of Genetics, Rutgers University
Gene expression has become an integral technology in a variety of academic and industrial laboratories. Whether the focus of an experiment is centered around a cluster of well-defined genes or more global profiling approach, a "model of consistency" should be established to ensure the minimization of technological variation. This tutorial will outline the development and implementation of standard practices for employing low, moderate and high throughput gene expression technologies in your laboratory. Topics and protocols for sample accessioning, nucleic acid extraction and amplification, technology selection and data analysis will be among the topics covered in the tutorial.
Who should attend:
Researchers, Clinicians, Core Directors, Graduate Students, Post-docs interested in learning about and implementing cutting edge gene expression technologies with a focus on sample preparation and microarray validation.
What shall be learned:
Principles for laboratory organization, sample tracking and processing, assay and experimental design, fundamentals of gene expression data analysis.
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