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Register by June 29th and Save up to $300!

September 16 - 8:30am-5:00pm

A one-day hands-on workshop focused on culturing mammalian cells including CHO and NSO.

  • Expansion culture, passaging, media, counting, storage/freezing
  • Tips and guidance for successful culture of the latest Eukaryotic host systems
  • Transformation techniques
  • Screening and purification techniques

Enrollment limited to 20 participants
Available only to registrants of the Optimizing Cell Culture Development meeting

*Separate Registration Required

Media Optimization Workshop *
Monday, September 17 - 9:00am to Noon

Join in this indepth focus on media including serum-free, serum-containing, and animal-component-free to help you develop the optimal conditions for growing mammalian cells. Experts will discuss nutrient demands as well as potential toxicities, and the effects of supplements on cell line adaptation. Media Optimization will be addressed for the most important cell lines used today including hybridoma, insect, Chinese Hamster Ovary (CHO), HEK 293 and Vero. 

Media Optimization -- the foundation for Optimizing Cell Culture Development! 

*Separate Registration Required


12:00pm Registration


1:30 Chairperson's Remarks 

1:45 Title to be Announced
Indresh K. Srivastava, Ph.D., Associate Director, Immunology and Cell Biology, Novartis Vaccines and Diagnostics, Inc.

2:30 Fractionation and Characterization of a Peptone Used in CHO Cell Culture Processes
Masaru Shiratori, Ph.D., Research Fellow, Late Stage Cell Culture Process Development, Genentech, Inc.
Peptones (protein hydrolysates) are an important media supplement for cell culture processes that do not exhibit optimal performance in chemically defined media. In many cell culture processes, peptones have been shown to enhance product titers as well as overall viable cell densities and culture longevity. Unfortunately, the composition of peptones is undefined and that contributes to possible disadvantages including: 1) process variability, 2) possible introduction of adventitious agents, and 3) the use of single-sourced raw materials by definition. Our goal is to identify and characterize the bioactive component or components in an animal derived peptone. The identification and understanding of novel bioactive components allows for the development of better defined and animal component free basal media and feeds. This presentation will focus on strategies and tools used to investigate complex mixtures including a small scale bioassay designed and implemented to assess the bioactivity of generated peptone fractions.

3:00 Development of a High-Producing PER.C6 Cell Culture Process
John Chon, Ph.D., Director, USP Development, PERCIVIA
One of the biggest challenges today in the biotechnology industry is to make low-cost biologics, despite bottlenecks such as low yields in both upstream and downstream operations. A process that is capable of producing large amounts of the product quickly is valuable not only for preclinical and early-phase clinical material generation but also in late-clinical and commercial manufacturing stages. This presentation will describe efforts at PERCIVIA, DSM, and Crucell in the development of such a production process. Through innovative use of existing technologies and taking advantage of the resilience of the PER.C6 cell line, we were able to achieve productivity greater than 10 g/L of monoclonal antibody in the space of less than 16 days without affecting product quality. The talk will discuss the strategy behind the process design and plans for further process optimization and scale-up, as well as integration with an efficient purification process able to handle high protein loads and the transfer of this high yield process into the GMP manufacturing environment.

3:30 Networking Refreshment Break in Exhibit Hall

4:05 Moderated Roundtable Discussions

5:15 Roundtable Report Out

5:30 Reception in the Exhibit Hall (Sponsorship Available)

6:30 End of Day One


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