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Biomarker Assay Development - Main Conference - Day 3

Conference Proceeding CD Now Available
  • Speaker Presentations
  • Poster Abstracts
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Day 1 | Day 2 | Day 3



8:00am Morning Coffee



8:25 Chairperson’s Opening Remarks

8:30 Image-Based Method for Measuring a Tissue Biomarker in Archived Clinical Samples

Marina Guvakova, Ph.D., Adjunct Assistant Professor and Senior Research Investigator, Department of Surgery, University of Pennsylvania

Standard assessment of protein expression in fixed tissue by immunohistochemistry (IHC) is not quantitative and hence, not well-suited for measuring biomarkers. We developed and validated a method for quantitative assessment of protein expression in clinical samples based on image analysis of immunohistologically stained tissues. Our uniplex or “one marker at a time” method has obvious advantages compared to conventional IHC assessment: (i) minimum FFPE tissue for analysis; (ii) eliminates pathologist’s bias (iii) results more standard and reproducible; and (iv) measures small but biologically meaningful differences as the continuous rather than the ordinal parameter.

9:00 Multi-Modality Nanoprobes for Molecular Imaging

Xiaohu Gao, Ph.D., Assistant Professor, Department of Bioengineering, University of Washington

Metal and semiconductor nanoparticles in the 1-10 nm size range are of considerable current interest, not only because of their unique size-dependent properties but also their dimensional similarities with biological macromolecules (e.g., nucleic acids and proteins). These similarities could allow an integration of nanotechnology and biology, leading to major advances in medical diagnostics, prognostics, and targeted therapeutics. In this talk, I discuss recent development of multifunctional nanostructures for multiplexed and quantitative biomarker analysis.

9:30 Seeing Renal Stress and Injury in Real-Time: The Ngal Reporter Mouse Detects the Response of Distal Tubular Segments in vivo

Jonathan M. Barasch, M.D., Ph.D., Associate Professor of Medicine, Associate Professor of Anatomy and Cell Biology, Columbia University Medical Center

Many proteins released into the biofluid have been proposed to act as surrogates of damaged organs, yet the basic characteristics that link most biomarkers with the damaged organ has not been described. NGAL (Lcn2) is an anti-microbial protein that is expressed in massive quantities by the kidney when damaged by ischemia, medications, or sepsis-lipopolysaccharide. To evaluate whether kidney expression of NGAL is related to the appearance of NGAL in the urine, we generated a NGAL-reporter mouse. Our data demonstrate that kidney NGAL expression is proportional to the extent of injury and to uNGAL in timing of appearance, intensity and reversibility. In addition, cell lines from the reporter mouse are useful for screening for toxicity and for medications which reverse kidney damage and inflammation.

10:00 Networking Coffee Break with Exhibit and Poster Viewing

11:00 What Do Your Biomarkers Do? Insights from Multiphoton Imaging

Robert Bacallao, M.D., Associate Professor, Medicine, Division of Nephrology, Department of Medicine, Indiana University

Two-photon confocal microscopy is an advanced imaging technique that has enabled researchers to observe cellular events with sub-cellular resolution. We have adapted this technology to perform imaging in live animals using surgical approaches to stabilize the animal and bring an organ of interest within range of an imaging objective. Utilizing these techniques we can evaluate cellular function in kidney, liver, skeletal muscle, and bone marrow. This technology can be used to perform ADME/Tox studies and identify cellular response to small molecules. This talk will show examples of this imaging technology that provides critical insights into cellular function in vivo.


Applications of MRI and PET Approaches for Assessing Cell Function

Thomas Bocan, Ph.D., Senior Director, Head of BioImaging Center, PharmaTherapeutics Research & Development, Pfizer, Inc.

Over the past decade, imaging hardware such as magnetic resonance imaging (MRI), and positron emission tomography (PET) have become more widespread and available for use in pre-clinical drug discovery research. Tools such as PET tracers are used to assess expression of the target in/on cells within defined tissue regions and applied for assessment of target organ distribution or binding at a specific target. Applications of FDG-, FLT-, FMISO and FPBR-PET and functional MRI or MRS are useful in assessing cellular function in response to specific stimuli or disease state. Combinations of the various imaging approaches can be utilized to study disease progression or drug efficacy/safety.



12:30 PANEL DISCUSSION: Trends in Imaging Technologies for Biomarker Studies in the Clinic

Moderator: Thomas Bocan, Ph.D., Senior Director, Head of BioImaging Center, Pfizer, Inc.

1:00 Close of Conference



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