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Quantitative PCR - Pre-Conference Short Courses

PRE-CONFERENCE SHORT COURSES*
SUNDAY, MARCH 15

1:30 pm Short Course 1 Registration

2:00-5:00 (SC 1) GETTING THE MOST OUT OF YOUR REAL-TIME PCR EXPERIMENTS
Sponsored by

Topics to be addressed in this course include:

Review of critical assay design parameters
Optimization of assay performance from experiment set-up to data analysis
Troubleshooting common problems that impact assay efficiency, reproducibility and sensitivity

Who Should Attend & Why: All levels of PCR users are encouraged to participate and will learn good laboratory practices to help you optimize your real-time PCR assays and effectively troubleshoot your results. This will be an interactive workshop - attendees are encouraged to bring questions, data files and problem examples with them.

Course Instructor: Marwan Alsarraj, Product Manager, Gene Expression Division, Bio Rad Laboratories

5:00 pm Short Course 2 Registration

5:30-8:30 (SC 2) HOW TO DO SUCCESSFUL GENE EXPRESSION ANALYSIS USING REAL-TIME PCR

In this short course, all aspects of the real-time PCR work flow will be covered, including:

RNA quality control, assay design and validation, selection of stably expressed reference genes, and experiment design
Data analysis using state-of- the art strategies, methods, and quantification models
Importing quality controls during the work flow
Insights into state-of-the art normalization using multiple reference genes, error propagation, and inter-run calibration
An introduction to biostatistical analysis of gene expression

Who Should Attend & Why: Both the first time real-time PCR user and the seasoned experimenter will learn state-of-the-art strategies, algorithms and validated work flow for real-time PCR gene expression analysis will benefit from this course.

Course Instructors:
Jo Vandesompele, Ph.D., Professor, Center for Medical Genetics, Ghent University Hospital
Jan Hellemans, Ph.D., Center for Medical Genetics, Ghent University Hospital

*Separate Registration Required