Digital Course: Getting the Most out of Your Real-Time PCR Experiments

Recorded at: XGen Congress  

Digital Course: Getting the Most out of Your Real-Time PCR Experiments

March 16, 2010

About this Product:
All levels of PCR users are encouraged to participate and will learn good laboratory practices to optimize real-time PCR assays and effectively troubleshoot results.

About the Conference:
Gone are the days of one-size-fits-all PCR. Through effective amplification and quantification of low levels of nucleic acids, real-time quantitative PCR (qPCR) and reverse transcription PCR (RT-qPCR) have revolutionized life science research. The newest generation of these exquisite enabling technologies are now being customized to the application. Learn from savvy, seasoned researchers as they share their real-world experiences, targets, tips, techniques, and results.

Agenda at a Glance: 

Topics addressed in this course include:

  • Review of critical assay design parameters
  • Optimization of assay performance from experiment set-up to data analysis
  • Troubleshooting common problems that impact assay efficiency, reproducibility and sensitivity
  • Introduction to MIQE - recommended guidelines for publication of qPCR experiments
  • High Resolution Melt Analysis: Power, Potential and Limitations


Frank BizouarnFrancisco Bizouarn, International Field Application Specialist, Gene Expression Division, Bio-Rad Laboratories 

Biography: Francisco Bizouarn is currently an International Field Application Specialist for the Bio-Rad's Gene Expression Division. His current focus is qPCR , sample prep and nucleic acid research techniques.


Carl FisherCarl Fisher, Ph.D., Product Manager, Gene Expression Division, Bio-Rad Laboratories 

Biography: Carl Fisher is the product manager for the CFX Real-time detection systems and related software at Bio-Rad Laboratories. He was previously a senior technical support consultant in the Molecular Biology and Chromatography groups at Bio-Rad, supporting conventional and real-time PCR, horizontal electrophoresis, gene transfer, and chromatographic systems and media. Prior to his arrival at Bio-Rad Laboratories, he was a Post-doctoral fellow in the Pathology Department at Brigham and Women’s Hospital, Harvard Medical School. He has extensive research and troubleshooting experience in the areas of Molecular Biology and Biochemistry.


About this Product:
3 Presentations
Over 215 slides
149 minutes
Single Copy - $345.00
Site License - $1,380.00

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