June 19, 2012
11:00 am – 12:30 pm EDT
Symposium Course Description: If you are a clinical, protein or basic research scientist interested in learning about the latest developments in the elucidation of the MAP/K and PI3K pathways you should attend this webinar.
During this webinar, you will hear from three well-known experts on topics including:
- The latest developments to measure the activation states and molecular interactions of the kinases comprising the mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K-AKT) signal transduction pathways which heavily influence the regulation of cell proliferation, differentiation, and survival
- The integrated analysis (genomic and proteomic) necessary to understand molecular mechanisms underlying cancer progression
- Recently released data from researchers investigating cancer biomarker techniques in signal transduction pathways
- Comparative analysis of In Vivo and In Vitro expression of multiple Kinases in the MAPK Pathway and heterodimeric interactions
There will also be ample time for discussion and questions.
In this application focused webinar you will learn about:
- novel technology in protein biomarker discovery and analysis and translational cancer genomics
- development of assays for the study of kinase signal transduction pathways showcasing an exciting new protein analysis technology for detection and quantitation
- Useful assay technology to measure proteins, phosphoproteins, cytokines, protein-DNA interactions, low-abundance proteins and analytes with weak and transient interactions
Who Should Attend:
Scientists and Managers working in Oncology, Biomarkers, Antibody and Kinase research.
Speaker #1 Information: Translational Genomics in Oncology: Insights into Cancer Biology and Precision Medicine
Presenter: Kelly A. Frazer, Ph.D. Professor Pediatrics and Division Chief of Genome Information Sciences at UC San Diego and Rady Children's Hospital firstname.lastname@example.org
1. Integrated analysis to understand molecular mechanisms underlying cancer progression
2. Genomic approaches for understanding the molecular basis of drug resistance in cancer cells
3. Targeted Sequencing in Clinical Cancer Research
Speaker #2 Information: Signal Transduction Pathways: Comparative Analysis Of In Vivo and In Vitro Expression of Multiple Kinases in the MAPK Pathway
Presenter: W. Matthew Dickerson, PhD., Senior Scientist, Applications Development, BioScale, Inc
The mitogen-activated proteinkinase (MAPK) and phosphoinositide 3-kinase (PI3K-AKT) cascades are signal transduction pathways which heavily influence the regulation of cell proliferation, differentiation, and survival, and as such, are implicated in a wide variety of cancers. The ability to measure the activation states and molecular interactions of the kinases comprising these pathways are crucial in the efficient and effective development of anti-cancer pharmaceuticals and therapeutics. This interactive scientific web symposium will cover:
- The utility of non-optical acoustic membrane microparticle (AMMP) technology to assess and quantify the activation state of several kinases (EGFR, MEK, ERK, p38, JNK, and AKT)
- Results of the analysis done on several human tumor cell lines, with or without stimulation of surface ligand receptors, and compared with lysates prepared from xenografted tumors derived from the same cell lines.
- Correlating measurement of kinases and receptors in tumor cell lines (in vitro) with the same cell line xenografts (in vivo)
- Elucidation of the minimum (<1,000) cell counts needed per assessment of the analyte in samples
- Little to no interference, cross talk or quenching effects from sample matrices, demonstrated in recovery studies utilizing recombinant analytes spiked into tumor and cell line lysates
- Analysis of kinase pathways completed within a single 96-well plate.
Speaker #3 Information: Assay Development for Translational Medicine Studies
Paul Goldsmith, PhD (Retired)
Head of Antibody and Protein Purification Unit, Center for Cancer Research, National Cancer Institute
Moderator Information: W. Matthew Dickerson, PhD., Senior Scientist, Applications Development, BioScale, Inc
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