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Next Generation Sequencing - Day 1



1:00 pm Conference Registration



Next-Generation Sequencing is alive, thriving, and driving discovery. As costs come down and ease increases, these new, massively parallel high-throughput sequencing platforms are infiltrating multiple aspects of traditional biological research. However, each next-generation sequencing platform best lends itself to specific sequencing goals. This Tech Expo showcases the next-generation sequencing platforms to help you make informed purchasing decisions. Sponsored Seminars Hosted by:

2:00 Chairperson’s Remarks

Kevin Davies, Ph.D., Editor-in-Chief, BioIT World

2:05 Applied Biosystems logo The SOLiD™ 3 System - Taking Next-Generation Sequencing to the Next Level 

Michael Rhodes, Ph.D., Product Applications Senior Manager, Genetic Analysis, High Throughput Discovery, Applied Biosystems
The new SOLiD™ 3 System achieves new milestones in throughput in excess of 20 Gb of mate paired sequence data from a single run and 30-40 Gb of demonstrated throughput in Applied Biosystems R&D labs. Maintaining high accuracy, improvements in read length and unique mate-pair library strategies, the news system enables expanding applications from whole genome resequencing and SNP discovery to miRNA profiling. This presentation will review various applications that the new system capabilities enable and discuss an example of whole genome transcript profiling in single cells and whole genome resequencing for SNP and structural rearrangement discovery.

Helicos logo2:35 Enabling True Biology with Helicos™ Single Molecule Sequencing

Patrice M. Milos, Ph.D., VP & CSO, Helicos BioSciences Corporation

Helicos True Single Molecule Sequencing (tSMS)™ provides a unique view of genome biology through the direct sequencing of cellular nucleic acids in an unbiased manner providing both quantitation and sequence information. Using a simple DNA sample preparation which requires no ligation or PCR amplification genomic DNA is sheared, tailed with polyA and readied for hybridization to a flow cell surface containing oligo dT for initiating the sequencing by synthesis reactions. To demonstrate the fidelity and scale of the HelicosTM Genetic Analysis System three bacterial genomes were sequenced, each of distinctly different genomic contents in single flow cell channels of the available 50 channels. We have extended our research to include a variety of genomic targets including candidate gene regions, yeast and C. elegans, all with similar accuracy and coverage. The ability of our single molecule sequencing platform to provide quantitative measurements of genome biology include research efforts in small RNA measurements, assessment of copy number variation of human samples and a simple method for quantitative assessment of the transcriptome, digital gene expression – all without the requirement of ligation or amplification – a hallmark for measuring the biology of cells.

3:05 Refreshment Break, Poster and Exhibit Viewing

Illumina logo3:30 The Illumina Genome Analyzer - Transforming Systems Biology

Abizar Lakdawalla, Ph.D., Senior Product Manager, Sequencing
Applications, Illumina

The Genome Analyzer next-generation sequencing system has transformed our understanding of genome variations, epigenomics, transcriptomics, and the interaction of proteins with DNA and RNA. A comprehensive description of the Genome Analyzer system will be presented with effective approaches to address broad systems biology questions. Strategies and tools derived from sequencing multiple human genomes, large numbers of transcriptomes, and extensive ChIP-Seq samples will be described to maximize the data and sample throughput with the simple and easy-to-use Genome Analyzer workflow.

Roche 454 logo4:15 Moving Next Generation Sequencing into the Clinical Research Market:
Timothy Harkins, Ph.D., Director, 454 Sequencing Roche Applied Science, Roche, Inc
The Genome Sequencer FLX is now generating the longest reads within the next gen market with over 1 million unique sequencing reads that are 400 to 500 base-pairs in length. With a fast instrument run time of 10 hours and the ability to quickly analyze the sequencing data, projects involving 1,000’s of samples are able to be processed readily. The projects that will be presented include:
1) Sequencing HIV to detect low frequency drug resistant mutations
2) HLA sequencing – the most known polymorphic regions within the human genome
3) Using NimbleGen Sequence-Capture arrays to sequence the whole human exome
4) Detecting novel pathogens in complex environmental samples

4:45 Interactive Panel Discussion with Sequencing Leaders

Moderator; Kevin Davies, Ph.D., Editor-in-Chief, BioIT World

The term “next-generation” has become the“now generation.” As the genome unit price of these next-gen platforms continues to tumble, excitement is growing about the scientific and commercial potential of third-generation sequencing systems, from single-molecule methods to nanopores to ‘nanoball’ service models. Here, leaders from established and emerging next-gen platform providers trade insights on the latest scientific and technological advances, and answer your questions.

GenomeQuest5:30 Reception Hosted by GenomeQuest

Preparing Your Enterprise for Next-Generation Science
Ron Ranauro, President & CEO, GenomeQuest, Inc.
Those acquiring next-generation sequencing instruments are faced with a dilemma for managing the deluge of data – choose between building their own in-house system or using low-data-volume desktop tools. A sequence data management platform provides the best of both choices for managing and analyzing massive volumes of sequence data, and gets researchers back to the science, faster. 

7:00 Close of Day

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