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Pre-Conference Short CoursesMonday, 23 • 9:00am to 12:00pm *
(SC1) Optimizing Media – Achieving Super Soup
To grow mammalian cells, researchers need to provide an optimal in vitro environment. The key feature of successful cell growth is the culture medium. ‘Achieving Super Soup’ requires finesse and know-how in order to combine the right ingredients at the right times under the right conditions to achieve high titers. This workshop will provide a foundation for optimizing cell culture media presented by real-world experts who will also tailor a portion of the course to fit concerns and challenges faced by the workshop participants.
9:00 Rationale for Monitoring Metabolic Parameters in Cell Culture Processes
Timothy Fawcett, Ph.D., Director, The BioTechnical Institute of Maryland, Inc.
Cell culturing conditions play an important role in providing a suitable environment for the growth and development of animal cells. As expectations for productivity and reproducibility increase, a closer examination of mammalian cell culture media components is necessary. Therefore, we will discuss key media components, their reason for inclusion in media and the positive and negative results of decay, and metabolic breakdown. A rationale for monitoring some key media constituents and metabolites will also be given along with suggestions to enhance culturing conditions and increase productivity.
9:45 Rational Design of Culture Media: Approaches for Speed and Productivity
Scott D. Storms, Ph.D., Consultant, Cell Culture Consulting LLC
Current design of cell culture media for bioproduction of recombinant therapeutics requires effective use of a diverse set of scientific and management disciplines. Cell line, culture media, and process all have a large impact on product yield and quality. They can also have strong interactions requiring good understanding of the each area and pragmatic development strategies integrating them for best results. Done correctly, this can lead to short development timelines while maximizing success. To achieve high culture performance in the limited timelines given for process development, different phases can performed concurrently and modern methods of optimization such as Design of Experiment can be effectively used. To further complicate matters, quality and regulatory issues must be considered throughout all development projects. When conducted in a rational and integrated way, culture media and process development can improve yields while meeting project deadlines.
10:30 Refreshment Break
11:00 Customizing Cell Culture Media and Reagents
Michael Hanson, Director, Media Division,Caisson Labs
Sourcing customized cell culture media and reagents can provide unique challenges for individual consumers. This talk will explore the feasibility of producing custom media as well as the cost and other considerations faced by end users. Case studies will be presented to demonstrate some of challenges of custom manufacturing and ways those challenges have been overcome.
• Custom Media Applications
• What can be Customized?
• Batch Sizes
• Turnaround Times
• Cost Analysis
• Case Studies
11:30 Interactive Discussion with Speakers -- What are the Issues you Run Into?
12:00 End of "Optimizing Media" Short Course
Dr. Fawcett earned his undergraduate degree in Biochemistry from the University of New Hampshire, and his doctorate in Biochemistry from Louisiana State University. Prior to joining BTI in May 2001, he was Training Center Manager of Life Technologies, Inc. / Invitrogen. There he wrote, developed, and presented workshops designed to teach medical doctors, principal investigators, and laboratory technicians in areas such as cell culture, recombinant DNA, and protein expression through a combination of lecture and laboratory exercises. Dr. Fawcett has investigated molecular responses to cellular stress at the National Institute on Aging, and the National Institutes of Health in the Laboratory on Gene Expression and Aging. In addition, he consults in the industry. As Director of The BioTechnical Institute of Maryland, Dr. Fawcett leads BTI's Workshop Program and assists with curriculum development.
Scott D Storms, Ph.D., has over twenty years experience in scientific management and has led culture media and strategy optimization projects for over 20 recombinant CHO cell lines. He currently is running a scientific consultancy practice where he advises and provides services to his clients for upstream process development for manufacture of biologics. Prior to this Scott was Director of Research and Development at Irvine Scientific for 8 years where he gained broad experience in the culture media business as the Design Control team leader for many custom and catalog products. He received his PhD in Biological Sciences from the University of California at Irvine where he currently sits on the Dean’s Leadership Council and received postdoctoral training at Case Western Reserve University in the Dept of Neurosciences.
Michael Hanson earned his B.S. degree in Biology from Brigham Young University-Idaho. For the past eight years he has worked with Caisson Laboratories in several capacities, including Director of Operations and currently as Director of Sales and Marketing. During his time at Caisson, Michael has been involved in numerous custom projects in both a manufacturing and sales role.
(SC2)Affinity Tag Purification Systems
Affinity tagging of recombinant proteins allows the purification of multiple proteins with one technique. In order to provide efficiency it is important to explore the limitations and opportunities, various generations of purification systems have to offer. This workshop provides the opportunity to learn about manual and automated purification systems, to assess the challenges and advantages involved and to discuss common problems during purification.
Short Course Topics:
David Wood, Ph.D., Associate Professor, Dept. of Chemical & Biomolecular Engineering, Ohio State University
Improved MBP Affinity Tags
Paul Riggs, Ph.D., Senior Scientist, Department of Research, New England BiolabsA Novel Purification Approach Based on HaloTag TechnologyRachel Friedman Ohana, Ph.D., Senior Research Scientist, PromegaHaloTag is a versatile protein fusion tag combining expression, detection and purification capabilities. This novel purification approach provides highly efficient protein purification through covalent immobilization coupled with proteolytic tag removal. HaloTag capabilities will be demonstrated through case studies of purifying multiple proteins from different expression systems.
Dinner Short Course • Tuesday, August 24 • 6:15pm to 9:00pm
(SC3)E.coli Innovations -- Includes a Plated Dinner
Escherichia colihas proven its worth as a protein expression platform. Currently, E.coli is not viewed so much as an ‘alternative’ platform, but as a viable choice for achieving high-level expression of human genes and protein. This Dinner Short Course will explore strategies for successful E.coli protein expression and process optimization.
6:15 Host Cell Engineering to Improve Product Quality
Kevin J. Rust, Principal Scientist, Pfizer, Inc.
In this talk, we will discuss the use of genetic methods to improve the quality of expressed peptide material. We will examine a method for making gene deletions, and provide specific examples of its implementation from past project work. We will examine the outcome of these genetic modifications on the quality of the process material generated. We will discuss the potential of genetics as a means to improve the quality of expressed peptides in future projects.
7:00 Dinner Break
7:30 Optimizing Expression in Escherichia coli: Conditions and Strains and Hosts, Oh My
Dominic Esposito, Ph.D., Principal Scientist, Protein Expression Lab, SAIC Frederick
Expression of proteins in E. coli requires optimization of a large number of variables in order to obtain the highest levels of good quality protein. As targets become more challenging, considerably more effort is required to find optimal conditions for expression. This talk will discuss many of the variables that can be optimized for improved expression, and ways to minimize the time and effort required to find the best conditions for a given protein. We will examine the effect of different host strains, conditions for expression, and the use of expression and solubility fusion tags on the production of large amounts of useful protein from E. coli.
8:15 'Last Ditch' Expression Optimization in E.coli: Unusual Tricks for Structural Studies of Phosphatases, Kinases and Toxins
Rebecca Page, Ph.D., Assistant Professor, Department of Molecular Biology, Cell Biology and Biochemistry, Brown University
I will be presenting some unusual methods that are used to successfully express eukaryotic and prokaryotic proteins that otherwise express insolubly (or not at all) in E. coli
9:00 End of E.coli Innovations Short Course
*Separate registration required
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