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Cambridge Healthtech Institute’s 4th Annual
Higher-Throughput Protein Purification
Supporting Technologies
January 22-23, 2015


High-throughput processes have transformed the traditional protein-by-protein trial-and-error approach for testing criteria and scaling up. This leading meeting on Higher-Throughput Protein Purification investigates HTP in the quest to develop methods that ensure quality and translate to large scale. Automation, robotics and liquid handlers will be discussed, along with developing small-scale models that shed light on bioproduction. Case studies illustrate how leaders in the field are integrating HTP approaches in order to reduce the time and effort needed to successfully establish parameters and achieve pure protein.


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Final Agenda 


THURSDAY, JANUARY 22

11:30 am Conference Registration

12:30 Luncheon Presentation (Sponsorship Opportunity Available) or Enjoy Lunch on Your Own

1:30 pm Ice Cream Break in the Exhibit Hall with Poster Viewing


HTP Automation

2:00 Chairperson’s Opening Remarks

Opher Gileadi, Ph.D., Principal Investigator, Genome Integrity and Repair, The Structural Genomics Consortium (SGC), University of Oxford


Keynote Presentation

2:05 Automated Higher-Throughput Protein Purification Using Enhanced ÄKTA Chromatography Instruments

Kenneth WalkerKenneth Walker, Ph.D., Scientific Director, Biologics, Amgen, Inc.

In order to keep pace with the increase in high-throughput cloning and expression needed to screen large panels of therapeutic candidates, we developed systems to substantially enhance the throughput of ÄKTA chromatography instruments through the use of novel large format autosamplers and quasi-parallel processing techniques. With little user intervention, these instruments can process a wide array of samples employing flexible, tandem, two column processing rapidly generating high quality products.


2:45 Acceleration of Purification Process Development Using High-Throughput Automation

Srinivas ChollangiSrinivas Chollangi, Ph.D., Scientist, Bristol-Myers Squibb Co.

By incorporating high-throughput (HT) technologies at key points in development, we show that an operating space for in-process conditions can be defined quickly and improve product and process knowledge prior to scale-up experiments. This allows for rapid process development of mAbs while minimizing the material requirement for early-stage development. As HT technologies continue to emerge, this approach will become increasingly powerful and will be applied to downstream process development of other biologics.

3:15 Automation of Non-Chromatographic Downstream Processes Gaining on High-Throughput Bioprocess Development

Cornelia WaltherCornelia Walther, Ph.D., Scientist, Applied Microbiology, University of Natural Resources and Life Sciences Vienna

We present an automated platform for parallel screening of inclusion body solubilization and protein refolding conditions using DoE. The incompatibility of numerous compounds in refolding buffers is overcome by elution of the captured protein in a single buffer. This platform enables the screening for optimized conditions of the entire protein recovery from inclusion bodies creating a holistic view on all crucial impact factors in an early stage of process development.

3:45 Sponsored Presentation (Opportunity Available)

4:15 Refreshment Break in the Exhibit Hall with Poster Viewing


HIGH-THROUGHPUT TECHNIQUES TO ENSURE QUALITY

5:00 A Fast, Automated, Quantitative, High-Throughput Platform for Detailed Glycan Analysis and Monitoring of Biotherapeutics by LC/MS/MS

Pauline M. RuddPauline M. Rudd, Ph.D., Professor, Glycobiology, National Institute for Bioprocessing Research and Training (NIBRT)

Monitoring glycosylation remains a challenge in the production of recombinant therapeutics. A new robotic HT platform for releasing and fast labelling N-glycans provides a front end to high resolution LC/MS/MS. Experimental data bases to aid LC data interpretation are open source (http://glycobase.nibrt.ie/tools.html). Databases and software designed for Pharma are incorporated into Waters UNIFI 1.7. Monosaccharide sequence and linkage information (LC) is directly compared with on line MS/MS for confident structural assignment.

5:30 Using High-Throughput Techniques to Produce Difficult Targets: Flavivirus NS1, a Case Study

William (Clay) BrownWilliam (Clay) Brown, Ph.D., Associate Research Scientist, Life Sciences Institute, University of Michigan

Flavivirus NS1 protein is a multi-functional protein that is required for genome replication and is involved in immune system evasion. These key roles in the Flavivirus infection cycle make NS1 an attractive target for drug and/or vaccine development. We applied high-throughput cloning and expression evaluation techniques for the identification of constructs that would be suitable for crystallization and 3-D structure determination. We also used a matrix buffer screen for initial purification development.

6:00-7:00 Reception at the Tiki Pavilion



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