Next Generation Dx Summit

Mass Spectrometry in Diagnostics of Infectious Disease 

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Recommended Pre-Conference Short Courses*

Introducing Mass Spectrometry into a Clinical Laboratory 


*Separate registration required


TUESDAY, AUGUST 21

1:00 pm Registration

 

Applying Mass Spec-Based Methods to Infectious Disease 

2:15 Chairperson’s Remarks

Robin Patel, M.D., Chair, Division of Clinical Microbiology, Consultant, Divisions of Clinical Microbiology and Infectious Diseases, Professor of Microbiology and Medicine, College of Medicine, Mayo Clinic

2:20 Progress and Approaches in Developing Mass Spectrometry-Based Proteomic Assays to Distinguish Clinical Strains of Methicillin-Sensitive and Methicillin-Resistant Staphylococcus aureus

Richard-DrakeRichard R. Drake, Ph.D., Professor and Director, MUSC Proteomics Center, Medical University of South Carolina

The use of matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry to identify bacteria at the genus and species level is becoming standard in many clinical microbiology laboratories. Extending these methods and development of other mass spectrometry-based diagnostics for identification of strain differences within a species, particularly for clinical isolates, remains a challenge. Our group has been focusing on assay development to distinguish methicillin-sensitive (MSSA) and methicillin-resistant (MRSA) Staphylococcus aureus strains. Two primary approaches will be described. One will cover progress in implementing and further testing of a prototype MALDI-TOF  three peak intensity model designed to rapidly identify USA300 family S. aureusclinical isolates. The other approach is a deeper proteomic mining comparison of different MSSA and MRSA clinical strains to identify a panel of strain-specific, differentially expressed proteins for use in further diagnostic assay development. 

2:50 Rapid Detection of Beta-Lactamase Activity in Clinical Specimens by Electrospray LC/MS/MS

Michael-HodsdonMichael E. Hodsdon, M.D., Ph.D., Associate Professor of Laboratory Medicine and Pharmacology, Yale University School of Medicine

Carbapenemase-expressing bacteria are associated with resistance to most modern antibiotics. Rapid diagnosis is critical to live-saving clinical care and to prevent the spread of outbreaks. We present a rapid, liquid chromatography-coupled, electrospray ionization mass spectrometric assay that detects carbapenemase activity within hours from complex biologic matrices. We are validating this method on both bacterial isolates and primary clinical specimens.

 

3:20 Sponsored Presentation (Opportunity Available)

3:35 Refreshment Break in the Exhibit Hall with Poster Viewing

4:10 Digging Deeper: Improving Results through Detailed Analysis of MALDI-TOF Data

Mark-FisherMark Fisher, Ph.D., Assistant Professor of Pathology, The University of Utah, ARUP Institute for Clinical and Experimental Pathology

MALDI-TOF is rapidly becoming an accepted identification method in the clinical microbiology laboratory. Current platforms accurately identify a broad range of organisms, but yield ambiguous results for some clinically relevant pathogens. Detailed analysis of the data at both the scoring and spectral levels can allow for better resolution and more reliable results.

 

4:40 Next Generation Molecular Diagnostics: MALDI-TOF versus PCR/ESI-MS

Robert-BonomoRobert Bonomo, M.D., Associate Professor of Medicine, Division of Infectious Disease and HIV Medicine, Case Western Reserve University

The field of molecular diagnostics is rapidly changing and new platforms are introduced regularly.  At present clinicians must decide the advantages and disadvantages of choosing between technologies that identify pathogens alone from technologies that reveal the resistance determinants present in bacteria.  Each approach assists with decisions to initiate therapy.  A comparison of technologies that use mass spectrometry (MALDI TOF and PCR/ESI-MS) will be examined.

 

5:10 Wine and Cheese Pairing Welcome Reception in the Exhibit Hall with Poster Viewing

6:10 Close of Day



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