New Method for Studying Quaternary Structure of GPCR Proteins In Vivo in Real Time

November 28th, 2012
11:30 am – 1:00 pm EST


Sponsored by
Aurora Spectral Technologies

Symposium Course Description: The course provides a summary of the development and utilization of a new spectral imaging system, OptiMiS™, to study quaternary protein structure in living cells in real time. The research will detail the use of this new system to determine the quaternary structure of a series of GPCR proteins in the membrane and cytoplasm of cells. Since the time for analysis is on the order of milliseconds, the structure of these proteins can be assessed in ‘real time’ before the impact of diffusion occurs.

Learning Objectives:

  • Learn about the structure of GPCR proteins in living cells
  • Assess the value of a new imaging methodology and system for measuring protein interactions in vivo 
  • Learn about a new spectral imaging system and its use as a tool in research on protein structures

Who Should Attend:

  • Research and Development Scientists
  • Directors or Managers of Research and Development
  • New Technology Specialists
  • Drug Discovery Scientists
  • Protein Biochemists

Program Agenda:

  • Introduction:
  • Dr. Valerica Raicu: 25 minutes
  • Dr. James Wells: 25 minutes
  • Dr. Graeme Millligan: 25 minutes
  • Dr. Mike Stoneman: 15 minutes
  • Summary

Speaker Information: 

Dr. Valerica Raicu, Ph.D.
Valerica RaicuChairman, Department of Physics
1900 E. Kenwood Blvd.
Milwaukee, WI 53211
Telephone: (414) 229-4969

Valerică Raicu holds a PhD degree in Biophysics from the University of Bucharest, Romania. Between 1991 and 2004, he has held research and academic positions at the Institute of Physical Chemistry of the Romanian Academy (Bucharest), Kochi Medical School (Japan), and University of Toronto (Canada). Since 2004, Raicu has been at the University of Wisconsin-Milwaukee, where he currently is an Associate Professor and Chair of the Department of Physics, and an adjunct faculty member in the Department of Biological Sciences. He is also the Chief Scientific Officer of Aurora Spectral Technology, LLC. Professor Raicu has authored or co-authored over forty papers and book chapters as well as a book ("Integrated Molecular and Cellular Biophysics,” Springer, 2008) on various topics in biophysics and biophotonics. He has also given numerous talks at scientific meetings and academic and research institutions all over the world.

Dr. James W. Wells, Ph.D.
James WellsProfessor
Leslie Dan Faculty of Pharmacy
144 College Street, Rm 906
Toronto, Ontario M5S 3M2
Telephone: (416) 978-3068

James W. Wells is a professor in the Department of Pharmaceutical Sciences and the Department of Pharmacology and Toxicology, University of Toronto. He received his undergraduate degree in pharmacy and his Ph.D. degree in biochemistry, both from the University of Toronto. Following postdoctoral training in the Division of Molecular Pharmacology at the National Institute for Medical Research in London, he returned to the University of Toronto to join the staff of the Leslie Dan Faculty of Pharmacy. His interest is in the mechanism of signalling via G protein-coupled receptors, with the M2 muscarinic cholinergic receptor taken as the case in point. His approach is based on the development of explicit mechanistic models, which are used to decode the data and to inform the design of pharmacological, biochemical and biophysical studies of preparations that range from single molecules to live cells. Of particular current interest is the status of the receptor as a monomer or an oligomer, which is studied in part by means of Förster resonance energy transfer.

Dr. Graeme Milligan, Ph.D.
Graeme MilliganProfessor of Molecular Pharmacology (Institute of Molecular Cell and Systems Biology)
Dean of Research (MVLS College Senior Management)
University of Glasgow
University Avenue
Glasgow, Lanarkshire G12 8QQ
United Kingdom
Telephone: 01413305557

Dr. Milligan’s research centers on the function, structure and regulation of G protein-coupled receptors (GPCRs) and their interacting proteins. He has published more than 400 peer-reviewed articles on these topics along with numerous reviews and other scholarly articles and his research has been cited more than 14,000 times. Dr. Milligan is a member of the scientific advisory board of 3 emerging biopharmaceutical companies that focus on the structure and function of GPCRs. He was elected to the Fellowship of the Royal Society of Edinburgh in 1998 and, amongst various honours, was the recipient of the 'Ariens' Award for Pharmacology from the Dutch Pharmacological Society in 2006.

Dr. Michael Stoneman
Research Scientist
Aurora Spectral Technologies
9100 N. White Oak Ln #229
Bayside, WI 53217
Telephone (414) 333-2111

Download Webinar  

By Series:
By Region: