Affinity tags are highly efficient tools for purifying proteins from crude extracts. To maintain a competitive edge, it is important to choose the right tag for the job and to address issues such as quality, efficiency and cost effectiveness. This conference address key questions such as: Which resins are optimal for which procedure? Do tags need to be removed and if so, how? What are possible validation steps to show that tags are not in the final product? What are the newest tagging technologies? Self cleaving vs. proteases, what are the differences and advantages? Is there an optimal way to ensure high yields, high speed and low costs?
SESSIONS
Tool and Tags
Purification Strategies
Cleavage
Preliminary Agenda
Alternatives to Tag-Based Affinity Purification
Jeffrey Culp, Ph.D., Associate Research Fellow, Primary Pharmacology Group, Research Centers of Emphasis, Pfizer Global R & D
Efficient Trapping and Purification of Target Protein Polyclonal Antibodies from GST- Protein Immune Sera
by an Epitope Affinity Column
Dan Crimmins, Ph.D., Senior Scientist, Pathology and Immunology, Division of Laboratory and Genomic Medicine, Washington University School of Medicine
Talk Title to be Announced
Demetrios Vavvas, M.D., Ph.D., Instructor, Ophthalmology, Massachusetts Eye & Ear Infirmary, Harvard University
Quality Control of Affinity-Purified Proteins
Speaker to be Announced
Multitag Affinity Purification of Human Membrane Receptors
Isabelle Mus-Veteau, Ph.D., Project Leader, Institute of Developmental Biology and Cancer, CNRS and University of Nice
Protein Purification with an Inducible Autoprocessing Enzyme Tag
Aimee Shen, Ph.D., Scientist, Department of Pathology, Stanford University School of Medicine
Talk Title to be Announced
Geoffrey S. Waldo, Ph.D., Team Leader, Biosciences, Los Alamos National Laboratory
Hexahistidine-Tag-Specific Optical Probes
Michael Fried, Ph.D., Associate Professor, Molecular and Cellular Biochemistry, University of Kentucky
Cleavable Affinity Tags - Challenges and Opportunities
William Gillette, Ph.D., Senior Scientist, Protein Purification Group Leader, Protein Expression Laboratory, Advanced Technology Program, SAIC-Frederick, Inc.