Gene expression in mammalian cells is the foundation for protein production and the creation of biologics. As more protein-based products head into development, the need to refine processes for optimizing cell line development increases. Reducing the time needed to identify high-expressing clones and achieve transfection is essential for trimming a project’s overall costs.
The Optimizing Mammalian Cell Lines meeting features experts sharing their protocols and case studies that illustrate how they have optimized cell lines to reach enhanced expression. Their insights and experiences will provide real-world details to help you conquer the challenges of cell line development. As you seek to enhance your skills and knowledge, make time to join the information exchange and network with colleagues from around the world who share common cellular goals on the path to cell line development.
Scientific AdvisorsSusan Dana Jones, Ph.D., Vice President and Senior Consultant, BioProcess Technology Consultants
Dennis Kraichely, Ph.D., Associate Director, CMC Team Lead, Portfolio Management & Technical Integration, Johnson & Johnson Pharmaceutical R&D, Inc.
Robin Ng, Ph.D., Senior Bioengineer, Cell Culture Process Development, Shire Human Genetic Therapies
Fubao Wang, Ph.D., Senior Research Fellow, Vaccine & Biologics Research, Merck & Co.
Preliminary Agenda
Opening Keynote Presentation:
Joseph Shiloach, Ph.D., Chief, Biotechnology Unit, National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), NIH
Rapid Development of CHEF1 Clonal Antibody Production Cell Lines
Howard Clarke, Ph.D., Principal Scientist, Process Development, CMC ICOS Biologics
Talk Title to be Announced
Bryan Hoffman, Ph.D., Project Manager, Global Project Management, Morphotek, Inc.
Developing CHO Host Cell Lines using the Artificial Chromosome Expression (ACE) Technology
Malcolm L. Kennard, Ph.D., Principal, Kennard Biologic Consultants
Optimized Transient Production of Recombinant Antibodies in HEK293 Cells for Research Applications
Thomas Schirrmann, Ph.D., Research Group Leader, Institute of Biochemistry & Biotechnology, Technical University of Braunschweig
Screening of Antibody Expressing Clones with an Automated Deep Well System
Benjamin Wang, Ph.D., Scientist, Process Cell Culture and Fermentation, MedImmune
Expression of Recombinant Proteins for Human Therapeutics Using the PER.C6 Human Cells
John H. Chon, Ph.D., Senior Director, BioProcess R&D, PERCIVIA, LLC
Talk Title to be Announced
Susanne Corisdeo, Research Scientist, Cell Biology, Centocor R&D Inc
Comparative Transfection of DNA into Primary and Transformed Mammalian Cells from Different Lineages
Dieter C. Gruenert, Ph.D., Senior Scientist, Head, Stem Cell Research Program, California Pacific Medical Center Research Institute; Professor, Department of Laboratory Medicine, University of California, San Francisco; Professor, Department of Medicine, University of Vermont
Talk Title to be Announced
Matthew H. Wilson, M.D., Ph.D., Assistant Professor, Department of Medicine, Nephrology Section, Michael E. DeBakey VA Medical Center, Baylor College of Medicine
Achieving Transfection Through Electroporation
Speaker to be Announced
Talk Title to be Announced
David J. Cappelleri, Ph.D., Assistant Professor, Mechnical Engineering, Stevens Institute of Technology