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2013 Archived Content

August 21-22, 2013

Cambridge Healthtech Institute’s 5th Annual
Optimizing Cell Line Development

Enhancing Expression


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Thursday, August 22

8:00 am Morning Coffee


Cell Line Technologies 

8:25 Chairperson’s Remarks

Paula Meleady, Ph.D., Senior Research Scientist and Programme Leader, National Institute for Cellular Biotechnology, Dublin City University

8:30 An NS0 Case Study: The Influence of Cell Culture Medium on Phenotypic Stability

Eileen M. HighamEileen M. Higham, Ph.D., Scientist II, Process Cell Culture & Fermentation, MedImmune - Biography 

 



9:00 Optimizing Transient and Stable Production Platforms for Antibodies and Other Proteins

Andreas-PoppAndreas Popp, Ph.D., Director, Protein Sciences; Head, Production, MorphoSys AG - Biography 

MorphoSys has implemented versatile manufacturing platforms based on different mammalian cell lines. Our presentation will focus on expression strategies to supply antibodies, antibody fragments, antigens and tool proteins during discovery, pre-clinical and clinical phases of therapeutic antibody development. Case studies on optimization of a high throughput compatible expression platform for small scale production of purified Fab and IgG materials as well as approaches to manufacture difficult-to-express proteins will be presented. 

9:30 Addressing Challenges for Expression and Purification of Human Multi-Protein Complexes Using the Baculovirus Expression System (BEVS)

Arnaud-PoterszmanArnaud Poterszman, Ph.D., Research Director, Integrated Structural Biology, Institute de Génétique, et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS - Biography 

We present here recent advances for the production of multi-subunit complexes in the baculovirus expression system using human multi-subunit transcription factors as model systems: Vector development for parallel expression/co-expression screening, use of fluorescent proteins as infection makers, manipulation of the phosphorylation pattern and construct design for production of large of multi-subunit complexes.

10:00 Coffee Break in the Exhibit Hall with Poster Viewing


Analytical Technologies 

10:45 High-Throughput Analytical Platforms for Cell Line and Process Development

Shashi PrajapatiShashi Prajapati, Ph.D., Senior Scientist, Cell Culture Development, Biogen Idec, Inc. - Biography 

Here we present various high-throughput (HTP) analytical platforms to facilitate rapid and parallel analyses of product quantity and quality using 96-well plate formats. These platforms include HTP protein quantitation followed by HTP protein purification and product quality analyses. With these analytical capabilities, we can assess product quality in the early stage of clone screening, as well as expedite the cell line and process development.

11:15 Vector Design Considerations for Monoclonal Antibody Production

Jan-SchoutenJan Schouten, Ph.D., Project Leader, BIO USP, Synthon BV - Biography 

A key component of the manufacturing process for recombinant antibodies is the actual production cell line itself. The foundation for a high-producing cell line is laid at the very early stage of expression vector design. Key choices are made then which have an impact that lasts throughout the entire product life cycle. Therefore it is critically important to address the parameters that contribute to the most optimal expression levels. This presentation will focus on the contribution of codon optimization strategies, the choice of leader peptides and other vector features. Also, attention will be paid to the different approaches possible to evaluate vector improvements in their most relevant context.

11:45 New Dimensions in Bio-Microscopy: Non-Invasive Live Cell Tomography

Yann-CotteYann Cotte, Ph.D., Research Scientist, Microvision and Microdiagnostics Group, EPFL School of Engineering (École Polytechnique Fédérale de Lausanne) - Biography 

Marker-free nanoscopy allows the ability to “see” the activity inside of a living cell in 3D. Using only harmless light, it enables observing the living cells label-free, non-invasively, and interference-free. The combination of holography with rotational scanning opens new dimensions for live cell monitoring. The cell’s tomographic reconstruction allows measuring cellular processes with real-time kinetics down to sub-cellular scale. Since the cells are not manipulated to introduce a label, it gives the possibility to measure the response of authentic cell’s physiological activity.

12:15 pm Luncheon Presentation (Sponsorship Opportunity Available) or Lunch on Your Own

1:00 End of Conference



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