Introduction To Cell Culture
August 20-21, 2014
Day 1: 9:00am – 5:15pm | Day 2: 8:30am – 12:00noon
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Timothy Fawcett, Ph.D., Scientific Director, BioTechnical Institute of Maryland, Inc., and Founder, BioSciConcepts
In this introductory seminar, we will discuss the delicate nature of animal cell growth, and how to develop a greater understanding of the science behind cell culture in order to avoid inconsistent results. Cell culturing conditions play an important role in providing a suitable environment for the growth and development of animal cells. Therefore, we will examine optimal conditions including pH, oxygen, light, temperature, media formulation, feed strategies, cell line identification, and safety.
This 1.5-day Intro to Cell Culture Training Seminar is a lecture based course intended for the beginner who is thinking about culturing animal cells for the first time or for intermediate cell culturists wanting to know more about how animal cell culture works and how to improve their process. Attendees will learn about most of the critical aspects of cell culture from equipment maintenance and media selection to cell growth and cryopreservation. Participants will have ample time to ask specific questions and get worthwhile answers.
Emphasis will be placed on how the cell culturist can reduce apoptosis by controlling the physical environment and the production of ammonia and free radicals through the optimization of the media formulation and the proper handling of the cells.
Along with looking at proper handling and optimal components, we will also focus on microscopy, as culturists should use the microscope frequently, making sure there is an understanding of the buffering systems used in culture and the general composition of traditional media.
In addition, we will discuss genetic drifting during culturing, and how being mindful of these cell line changes help us to provide the most accurate results possible for our own knowledge and also for publication purposes.
Topics to be discussed:
- Introduction to Cell Culture
- Equipment use and decontamination
- Biological safety cabinets and CO2 incubators
- Contamination prevention and types of contamination
- Cell Culture Media I and II
- Cell verification maintenance and storage
- Cell types, microscopy and confluency
- Transfection technology
- Clonal isolation of animal cells
- Primary culture and animal cell attachment and signaling
- Growth curves, growth strategies for growing animal cells in culture
Timothy Fawcett, Ph.D., Scientific Director, BioTechnical Institute of Maryland, Inc.
Timothy Fawcett, Ph.D., has been in the biotechnology business for over 30 years. Trained as a biochemist, he has held senior positions in both academics and industry and has been a mentor to many young scientists throughout his career. For the last 13 years, Dr. Fawcett has been the Director of the BioTechnical Institute of Maryland (BTI), a non-profit institute located in Baltimore, Maryland. He is also the Founder and Director of BioSciConcepts, a social venture of BTI that provides hands-on training for professional scientists in cell culture, baculovirus-based expression, as well as topics such as molecular biology, PCR and real-time PCR. BioSciConcepts is an internationally recognized provider of expertise in cell culture and the biological sciences and has provided consultation services to several small and large biotechnology companies. Dr. Fawcett has a deep knowledge of biotechnology and has experience in most of the technical aspects of the workflow.
Dr. Fawcett earned his undergraduate degree in Biochemistry from the University of New Hampshire, and his doctorate in Biochemistry from Louisiana State University. Prior to joining BTI in May 2001, he was Training Center Manager of Life Technologies, Inc./ Invitrogen. There, he wrote, developed, and taught workshops designed to teach medical doctors, principal investigators, and laboratory technicians in areas such as cell culture, recombinant DNA, and protein expression through a combination of lecture and laboratory exercises. Dr. Fawcett has investigated molecular responses to cellular stress at the National Institute on Aging, and the National Institutes of Health in the Laboratory on Gene Expression and Aging. In addition, he consults in the industry.