2013 Archived Content

SC10 Digital PCR Applications and Advances  


4:30 Opening Remarks and Speaker Introdictions

4:40 Scaling Up Digital PCR for High-Performance Diagnostics

Carl Lars Hansen, Ph.D., Assistant Professor, Center for High-Throughput Biology, Department of Physics and Astronomy, University of British Columbia

Digital PCR is a method for absolute quantification of nucleic acids through compartmentalization at limiting dilution followed by single molecule amplification and endpoint detection.  I will introduce the basic principals of digital PCR and highlight its use applications that require very high specificity, sensitivity, and precision. I will then present an implementation of very high density digital PCR, called Megapixel Digital PCR, that performs millions of pL volume amplification reactions on a microfluidic chip format and discuss the use of this technology for single cell gene expression measurements.

5:30 Break for Dinner

6:00 Digital Detection of Ultra-Rare Nuclear and Mitochondrial Mutations:  Fundamental and Clinical Implications in Cancer and Disease

Jason H. Bielas, Ph.D., Assistant Member, Molecular Diagnostics Program, Fred Hutchinson Cancer Research Center (FHCRC); Affiliate Assistant Professorship, Department of Pathology, University of Washington

6:45 Digital PCR and BEAMing: Applications and Implications for Breast Cancer Diagnostics and Therapy

Ben Ho Park, M.D., Ph.D., Associate Professor, Oncology, Breast Cancer Research Program; Associate Director, Hematology/Oncology Fellowship Training Program, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins

This talk will focus on the use of BEAMing for PIK3CA mutations in breast cancer for diagnostic purposes and to follow responses to therapy. Data from a prospective trial in metastatic breast cancer patients will be presented.

7:30        Close of Course

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