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SC6: Filtration – An Important Step in Protein Purification

Tuesday, January 22, 2013

4:30 - 7:30pm

 

4:30  Impact of Ultrafiltration/Diafiltration (UF/DF) Operation on Product Quality

Wei WangWei Wang, Ph.D., Research Fellow, Pharmaceutical R&D, Pfizer Global R&D

This presentation will briefly introduce the basic UF/DF set-up and operation, and describe operation challenges and possible solutions. Detailed discussion will be on interaction of a biological product candidate with UF/DF system leachables, and its impact on product quality.

  • UF/DF operation is used for many process applications;
  • Many challenges/issues are associated with the operation;
  • It is a critical step in preparation of biological products; and
  • Product quality can be impacted through interactions between a drug candidate and the UF/DF components.

 

5:45 Dinner Break

 

6:30 Integrated Bioprocess Development for Recombinant Protein Production and Purification

C. Perry ChouC. Perry Chou, Ph.D., P.Eng., Professor, Canada Research Chair in Biomanufacturing, Chemical Engineering and Biology, University of Waterloo

Bioprocess development for recombinant protein production generally constitutes three main stages, i.e., upstream of strain construction, midstream of cell cultivation, and downstream of protein harvest and purification. Conventional approaches for bioprocess development are often “fragmented” and lead to ineffective operation, as the three stages are independently studied. Technological cross-influence among the three bioprocess stages has now been widely recognized. As a result, all bioprocess parameters have to be systematically considered for novel operation with optimal performance. In this course section, strategies for such “integrated” bioprocess development will be elaborated. Also, a recently developed bioprocess for recombinant protein production in Escherichia coli will be used for strategic demonstration. Based on a novel downstream setup for simultaneous tangential-flow filtration and membrane chromatography, the produced recombinant protein can be readily harvested and purified along with cell clarification.

7:30 Close of “Filtration” Short Course

 

 Speaker Biographies: 

Wei Wang, Ph.D., Research Fellow, Pharmaceutical R&D, Pfizer Global R&D 

Wei Wang, Ph.D. is currently a Research Fellow in BioTherapeutics R&D at Pfizer Inc. He is an adjunct professor at the School of Pharmacy, University of the Pacific, St Louis College of Pharmacy, and a guest professor at Shandong University.  Wei got his undergraduate degree in Pharmacy in China and his Ph.D. degree in Pharmaceutical Sciences at the University of Southern California. Over the past 20 years, he has worked in several pharmaceutical companies, and has been directly involved in the development of a variety of parenteral and ophthalmic products, delivery systems, and vaccines, covering small molecules, peptides, proteins, oligonucleotides, and DNAs.  He has authored/co-authored more than 60 publications, patents, and presentations.

C. Perry Chou, Ph.D., P.Eng., Professor, Canada Research Chair in Biomanufacturing, Chemical Engineering and Biology, University of Waterloo 

C. Perry Chou received his BSc and MSc from National Taiwan University (Taipei, Taiwan) and PhD from Rice University (Houston, USA), all in Chemical Engineering. He is currently a full professor in Chemical Engineering Department at University of Waterloo (Waterloo, Canada) and an awardee of Canada Research Chair in biomanufacturing. He has been serving as an editor for Biotechnology Advances (Elsevier) since 2007. He primarily works on developing novel strategies to enhance the manufacturing of bioproducts of interest through the integrative application of bioprocess engineering, molecular biology, and microbial biotechnology.