SC1: Protein Charge Measurement: Why, What and How
Sunday, January 20, 2013
5:00 - 8:00pm
Having a sufficiently high protein charge is central to the development of stable, low-viscosity solutions. There are many misconceptions about protein charge: what constitutes a protein's charge and how charge is affected by the temperature and the solvent. Most importantly, protein charge cannot be calculated with any certainty, but it can be measured accurately. This workshop will introduce you to the different ways protein charge can be measured, to the advantages and disadvantages of each method and to some of the surprising results.
5:00 Presentation 1: Why Measure Protein Charge?
- The proximity energy framework for high concentration protein solutions
5:30 Presentation 2: What Exactly Constitutes Protein Charge?
- The ways to describe protein charge
- What contributes to the protein charge
- Solvent effects and manipulating protein charge
6:00 Dinner Break
6:30 Presentation 3: How Can Protein Charge be Measured?
- Electrophoresis... just enough theory to see you through
- Protein charge measurement by electrophoresis
- Best practices
- Some examples of protein charge measurements
8:00 Close of Short Course
Thomas Laue, Ph.D., Professor, Biochemistry and Molecular Biology; Director, Biomolecular Interaction Technologies Center (BITC), University of New Hampshire
Tom Laue is the Carpenter Professor of Molecular, Cellular and Biomedical Sciences, and professor of Material Sciences at the University of New Hampshire. He is the Director of both the Center to Advance Molecular Interaction Science and the Biomolecular Interaction Technologies Center. He received his bachelor’s degree in Natural Sciences from the Johns Hopkins University in 1971and his Ph.D. in Biophysics and Biochemistry from the University of Connecticut in 1981. His post-doctoral studies were conducted at the University of Oklahoma. Between 1969 and 1975, he worked as a technician in the deep space program of NASA. He joined the University of New Hampshire in 1984 as an Assistant Professor, and teaches both undergraduate and graduate courses in biochemistry and biophysics. His research focuses on the development of instrumentation and methods that use the fundamental properties of mass and charge for examining macromolecular interactions. His instruments can provide unique insights into these interactions, which has resulted in extensive collaborations with both academic and industrial labs. Tom has over 120 publications, serves on several editorial boards, and gives over one hundred lectures, seminars and workshops a year.