PTK 2015 Banner 
2015 Peptalk M Banner 

 

SC8: Transient Protein Production in Mammalian Cells 

Tuesday January 22, 2013 

Detailed Agenda 

 

 

4:30 pm Opening Remarks

Richard Altman, M.S., Research Scientist, Alexion Pharmaceuticals

4:40 Protein Expression Systems Overview

Dominic Esposito, Ph.D., Director, Protein Expression Laboratory, SAIC-Frederick, Inc.

• Advantages/Disadvantages of available systems
• Mammalian expression options

4:50 Fundamental Elements of Transient Protein Production

Dominic Esposito, Ph.D., Director, Protein Expression Laboratory, SAIC-Frederick, Inc.
• Expression Vectors


5:30 Dinner and Networking


6:00 Fundamental Elements of Transient Protein Production

Henry C. Chiou, Ph.D., Senior Manager, Molecular Biology, Life Technologies Corporation

• Cell lines
• Media and Cell culture
• Transfection reagents and protocols
• Strategies for maximizing yields

6:40 Implementing Transient Protein Production

Richard Altman, M.S., Research Scientist, Alexion Pharmaceuticals

• What is your goal/objective for transient protein expression?
• Case Study

7:15 Interactive Discussion


7:30 Close of Transient Protein Production in Mammalian Cells Short Course
 

 

Speaker Bio's 

Richard Altman, M.S., Research Scientist, Alexion Pharmaceuticals 

Richard AltmanMr. Altman has over twenty three years of experience working in the pharmaceutical industry.  For the past two years, he has worked in the Molecular Sciences group at Alexion Pharmaceuticals developing antibody and non-antibody biologics.  Prior to Alexion, he worked for several pharmaceutical companies on the cloning, expression, purification, and characterization of recombinant proteins. This work supported both high throughput screening and protein therapeutic efforts. In addition, he has been involved in the generation of transgenic animal models and their characterization.  Mr. Altman received his M.S. degree from the University of Pittsburgh School of Medicine in the Department of Molecular Biology and Biochemistry.

Dominic Esposito, Ph.D., Director, Protein Expression Laboratory, SAIC-Frederick, Inc. 

Dominic Esposito Dr. Esposito is currently the Director of the Protein Expression Laboratory (PEL) at SAIC-Frederick, Inc.  The 26 employees in the PEL generate proteins of interest to investigators at the National Cancer Institute and other NIH facilities, and invent and develop new technologies for protein expression and production.  Prior to his role as director, Dr. Esposito led the Clone Optimization Group in the PEL for 9 years and was responsible for the generation of over 15,000 expression clones, 400 new expression vectors, and several technological innovations in protein expression. Dr. Esposito received his B.A. in Chemistry at La Salle University in Philadelphia, and his Ph.D. in Biochemistry at the Johns Hopkins University Bloomberg School. Dr. Esposito previously worked for Life Technologies, where he helped to develop the Gateway recombinational cloning system.

Henry C. Chiou, Ph.D., Senior Manager, Molecular Biology, Life Technologies Corporation 

Henry ChiouDr. Chiou has over 15 years of experience working on development of mammalian protein expression systems and transfection reagents. For the past 9 years he has worked as a principal scientist and now in product development and portfolio management for Life Technologies. He has led the development efforts on a number of products, including the FreeStyle™ transient expression systems, Lipofectamine™ LTX and Lipofectamine™ RNAiMAX. Prior to Life Technologies, he worked for several biotech companies on biotherapeutic gene delivery systems. Dr. Chiou received his doctorate from Harvard University and completed a post-doctoral fellowship at the University of Pennsylvania.