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SC4: Protein Production in the Baculovirus/Insect Cell Expression System 


Sunday January 20th , 2013 

Detailed Agenda 

 

5:00 Opening Remarks

Dominic Esposito, Ph.D., Director, Protein Expression Laboratory, SAIC-Frederick, Inc.

5:10 Fundamentals of the Baculovirus/Insect Cell Expression System

Donald L. Jarvis, Ph.D., Professor, Molecular Biology, University of Wyoming

• The history of insect cells as an expression system
• What you need to carry out insect cell expression in your lab
• Development of vector technologies for baculovirus expression


5:50 Dinner and Networking


6:20 Advances in Insect Cell Technology for Recombinant Protein Production

Dominic Esposito, Ph.D., Director, Protein Expression Laboratory, SAIC-Frederick, Inc.

• Advances in vector technologies to increase throughput and lower cost
• Optimizing expression and purification strategies for insect cell expression


Donald L. Jarvis, Ph.D., Professor, Molecular Biology, University of Wyoming

• Humanizing protein glycosylation in insect cells


7:20 Development of Therapeutic Proteins and Vaccines in the Insect Cell System

James M. Groarke, Ph.D., Chief Scientific Officer, Biortus Biosciences.

• Historical perspective 
• Baculovirus vectors for vaccines &gene therapy
• Case studies

8:00 Close of Short Course

 

Speaker Bios 

 

Dominic Esposito, Ph.D., Director, Protein Expression Laboratory, SAIC-Frederick, Inc. 

Dominic Esposito Dr. Esposito is currently the Director of the Protein Expression Laboratory (PEL) at SAIC-Frederick, Inc.  The 26 employees in the PEL generate proteins of interest to investigators at the National Cancer Institute and other NIH facilities, and invent and develop new technologies for protein expression and production.  Prior to his role as director, Dr. Esposito led the Clone Optimization Group in the PEL for 9 years and was responsible for the generation of over 15,000 expression clones, 400 new expression vectors, and several technological innovations in protein expression. Dr. Esposito received his B.A. in Chemistry at La Salle University in Philadelphia, and his Ph.D. in Biochemistry at the Johns Hopkins University Bloomberg School. Dr. Esposito previously worked for Life Technologies, where he helped to develop the Gateway recombinational cloning system.

James M. Groarke, Ph.D., Biortus Biosciences 

James GroarkeDr. James Groarke, an expert in his field, has over 20 years of experience in a leadership capacity within the pharmaceutical and drug development arena.  The depth of experience gained in both large pharmaceutical corporations and small biotech companies has provided him with a comprehensive knowledge of the growing biotech industry in the United States and in Europe. James Groarke received his Bachelor’s degree in Biochemistry from New York University.  He then went on to attain his Ph.D. in Biochemistry and Molecular Biology from Purdue University.  At this time, James was offered a post-doctoral appointment at the University of California Berkley, but truly started his pharmaceutical career when he was appointed as a Senior Scientist at Sterling-Winthrop (now Sanofi-Aventis).  He was co-founder of the biotechnology company,ViroPharma, Inc and more recently, James was a senior investigator at Novartis for eight years before joining Biortus. James has authored over 50 publications and presentations and has made significant contributions in major disease areas, primarily within infectious diseases and oncology.  He is also recognized as the world expert in the Baculovirus Protein Expression system.  He joined Biortus Biosciences in 2011 as co-founder and Chief Scientific Officer and currently travels between our Carlsbad (California) and Wuxi (China) offices providing his expertise and extensive knowledge wherever possible.

Donald L. Jarvis, Ph.D., Professor, Molecular Biology, University of Wyoming 

Donald JarvisDon Jarvis earned B.S. (1978) and M.S. (1980) degrees in Microbiology at Idaho State University and a Ph.D. (1986) in Virology at Baylor College of Medicine. After a brief postdoctoral training period at Baylor, Don moved to Texas A&M University in 1987 to study glycoprotein biosynthesis and processing in the baculovirus system as a postdoctoral scientist in Max Summers’ group. In 1989, Don took an independent position at Texas A&M and continued to work in the baculovirus system until the end of 1997. Don subsequently moved to the Department of Molecular Biology at the University of Wyoming, wherehe has continued to develop a research program focused on glycoprotein biosynthesis in the baculovirus-insect cell system.