SC5: How to Obtain Reliable Information from Light Scattering: Theory, Practical Advice and Data Interpretation
Tuesday, January 22, 2013
4:30 - 7:30 pm
Dynamic light scattering (DLS), in particular, has occupied a position of increasing popularity within the area of protein aggregate detection and characterization, due in large part to the non-invasiveness of the technique, the minimal sample volume & concentration requirements, and the ease-of-use as well as speed of data collection. While modern instrument design and software have removed much of the barriers traditionally associated with the technique, data interpretation is still an area of frustration for many DLS users. This workshop covers the basic theory behind DLS instrumentation and multi-detector SEC, with a focus on the do’s & don’ts when it comes to data interpretation for biophysical characterization.
4:30 Welcome and Introduction to Faculty
Poll and interview of attendees to gauge backgrounds, level of experience, purposes for attending and desired takeaways
Presentation Part 1: DLS and multi-detector SEC: Theory & Data Interpretation with Interactive Q&A
- Dynamic Light Scattering – Hydrodynamic Mean Size & Polydispersity – Intensity-Volume-Number Distributions – Accuracy, Precision, Resolution – Common Issues & Frustrations – Data Interpretation Tools – Multi-detector SEC-LS
6:00 Dinner buffet and discussions over dinner
Presentation Part 2: Working DLS and SEC Examples with Interactive Q&A
- Data Interpretation Case Studies – Working with DLS data in the lab – Making sense of multi-detector chromatograms with (and without) DLS
Closing Quiz, Questions and Remarks, Final Take Home Messages
7:30 Close of Short Course
Kevin Mattison, Ph.D., Principal Scientist Bioanalytics, Malvern Instruments
Kevin completed his doctorate in biological chemistry at Purdue University, where he studied the effects of polyelectrolyte additives on the stability and activity of transport proteins and enzymes. From there he joined Protein Solutions as the Applications Development and Technical Support Manager, and was instrumental in helping to drive the adoption of sub-micron light scattering techniques from esoteric technologies into main stream laboratory tools. In 2002 Kevin joined Malvern Instruments, where he served as Applications Manager, Product Manager, and Director of Customer Support, prior to assuming his current position as Principal Scientist – Bioanalytics in the Strategic Technology Development Group.
Ulf Nobbmann, Ph.D., Prodcut Manager, GPC/SEC Technologies, Malvern
Ulf received his PhD in colloidal physics from Oklahoma State University, where he investigated the scattering of light from micron & sub-micron sized particle systems under both dilute and concentrated conditions. Upon graduation, he joined Protein Solutions, where he turned his attention to the application of colloidal principles and characterization techniques to biological molecules and nano particles. Ulf has written book chapters and scientific articles covering relevant aspects and applications in industry and biotechnology. He joined Malvern Instruments in 2003 in the UK and is currently Product Manager (Americas) for GPC/SEC Technologies.
Mark Pothecary, Ph.D., Product Manager for Light Scattering Products, Malvern Instruments
Mark studied for an undergraduate degree in Biochemistry at the University of Bath graduating in 2002. He went on to Queen Mary College at the University of London, to work on his Masters and PhD also in Biochemistry, which was granted in 2007. The subject of this work was to study the effects of red wine polyphenols on the production of nitric oxide within the cardiovascular system. A post-doctoral position followed where SPE and reverse-phase HPLC were used to purify these polyphenols from blood to study their bioavailability. In 2008 Mark joined Malvern Instruments as Product Technical Specialist for separation sciences working on Malvern's SEC solutions. In 2011 he was promoted to Product Manager for the light scattering products, including the Zetasizer and Viscotek products.