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CONFERENCE SERIES: Bioprocess and Manufacturing

Recorded at: PepTalk: The Protein Science Week

Digital Course: Strategies for Protein Purification and Overcoming Aggregation

 

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About this Product:

Together with input from Sarah Bondos from Texas A&M University, Mario Lebendiker of the Wolfson Centre for Applied Structural Biology at The Hebrew University of Jerusalem provides an indepth overview of protein purification with a particular focus on aggregation problems. This course reviews:

  • common causes of protein aggregation during expression and purification,
  • methods to detect the presence of aggregates,
  • the types and properties of chemicals that, when added to buffers, may improve protein solubility, and
  • methods to screen these additives to rapidly optimize the solubility of your protein.
 

 

Product Details:
1 Presentation
78 Slides
100 Minutes
Individual: $345
Site License: $1380

Formats Available:
Digital Download
On Demand
DVD
 

 

Agenda at a Glance:

Buffer Optimization for Purifying Protein

Mario Lebendiker, Ph.D., Head, Protein Purification Facility, Wolfson Centre for Applied Structural Biology, The Hebrew University of Jerusalem

This short course will review (i) common causes of protein aggregation during expression and purification, (ii) methods to detect the presence of aggregates, (iii) the types and properties of chemicals that, when added to buffers, may improve protein solubility, and (iv) finally methods to screen these additives to rapidly optimize the solubility of your protein.

Speaker Biography:

Mario LebendikerMario Lebendiker, Ph.D., Head, Protein Purification Facility, Wolfson Centre for Applied Structural Biology, The Hebrew University of Jerusalem

Dr. Mario Lebendiker is in charge of the Protein Purification Facilities at the Wolfson Centre for Applied Structural Biology, The Hebrew University of Jerusalem. He is actively involved in many collaborations for structural and biochemical studies within the Hebrew University, others Universities in Israel, as well as with biotech and pharmaceutical companies. Dr. Lebendiker received a PhD in Biochemistry in 1982 from the Animal Virology Center (CEVAN), in Buenos Aires University, Argentina. Together with many other laboratories, he found the Protein Production and Purification Partnership in Europe (P4EU) network; a platform for the exchange of information, know-how and materials between core facility labs in the field of protein expression and purification.

About the Conference:

CHI's PepTalk: The Protein Science Week is more than just a conference. It is a community dedicated to the evolving field of biotherapeutics and a gateway to the future of biologics. PepTalk centers around Five Protein related Pipelines: Formulation, Purification, Biotherapeutics, Expression, and Delivery. These synergistic piplelines cover biololgics from upstream discovery research to downstream clinical products.