January 13 - 17, 2014
Renaissance Hotel and Palm Springs Convention Center Palm Springs, California

A Community Dedicated to the
Evolving Field and Future of Biotherapeutics
2014 Archived Content

SHORT COURSE 12: TRANSIENT PROTEIN PRODUCTION
IN MAMMALIAN CELLS

 

Tuesday, January 14 | 5:00-8:00 pm



5:00 pm Opening Remarks

Richard Altman, MS, Research Scientist, Alexion Pharmaceuticals  

  • Advantages/Disadvantages of available systems
  • Mammalian expression options

 

5:10 Fundamental Elements of Transient Protein Production

Richard Altman, MS, Research Scientist, Alexion Pharmaceuticals  

  • Expression vectors

 

5:25 Fundamental Elements of Transient Protein Production

Henry C. Chiou, Ph.D., Senior Product Manager, Life Technologies  

  • Cell lines
  • Media and cell culture
  • Transfection reagents and protocols
  • Strategies for maximizing yields

 

6:15 Dinner

 

6:20 Implementing Transient Protein Production

Richard Altman, MS, Research Scientist, Alexion Pharmaceuticals  

  • What is your goal/objective for transient protein expression?
  • Case study

 

6:55 Implementing Transient Protein Production

Krista Johnson, MSc, Research Scientist, Alexion Pharmaceuticals  

  • Tools and strategies for purification of transiently expressed proteins
  • Methods of evaluation of purified proteins from transient expression

 

7:30 Interactive Discussion on Expression/Purification Troubleshooting  

8:00 Close of Transient Protein Production in Mammalian Cells Short Course  

 

Speaker Biographies:

Richard Altman, MS, Research Scientist, Alexion Pharmaceuticals

Richard AltmanMr. Altman has over 23 years of experience working in the pharmaceutical industry. For the past two years, he has worked in the Molecular Sciences group at Alexion Pharmaceuticals developing antibody and non-antibody biologics. Prior to Alexion, he worked for several pharmaceutical companies on the cloning, expression, purification and characterization of recombinant proteins. This work supported both high-throughput screening and protein therapeutic efforts. In addition, he has been involved in the generation of transgenic animal models and their characterization. Mr. Altman received his MS degree from the University of Pittsburgh School of Medicine in the Department of Molecular Biology and Biochemistry.

Henry C. Chiou, Ph.D., Senior Product Manager, Life Technologies

Henry ChiouDr. Chiou has over 15 years of experience working on development of mammalian protein expression systems and transfection reagents. For the past nine years he has worked as a principal scientist and now in product development and portfolio management for Life Technologies. He has led the development efforts on a number of products, including the FreeStyle™ transient expression systems, Lipofectamine™ LTX and Lipofectamine™ RNAiMAX. Prior to Life Technologies, he worked for several biotech companies on biotherapeutic gene delivery systems. Dr. Chiou received his doctorate from Harvard University and completed a postdoctoral fellowship at the University of Pennsylvania.

Krista Johnson, MSc, Research Scientist, Alexion Pharmaceuticals

Krista JohnsonKrista Johnson has been in the biotech industry for 20 years, working on all aspects of antibody therapeutic development. She has been purifying and characterizing antibodies and recombinant proteins for in vivo and in vitro use for over 10 years as part of the research and development team at Alexion. She was on the team that discovered and developed Soliris (eculizumab), a first-in-class terminal complement inhibitor. Krista holds a BSc in chemistry and obtained her MSc degree in pharmacology from Dalhousie University in Canada.



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 Protein Simple  

  

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Training Seminars 

Mon-Tues, January 13-14 

Biologics Formulation and Delivery  

 


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