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Cambridge Healthtech Institute’s 7th Annual
Engineering Genes, Vectors, Constructs and Clones
Upstream Decisions Lead to Downstream Success
January 19-20, 2015


Protein expression is never a simple task and the demand for high-quality biotherapeutic proteins continues to grow. Bottlenecks arise frequently because functional recombinant proteins are difficult to produce, thus protein expression engineers are often forced to return to the drawing board. This usually requires designing new cloning schemes, including lengthy verification and sequence analysis of the gene or protein of interest, moving a gene from one vector to another, transfecting the vector in an alternative host, re-characterizing the expressed protein or any of the above—an inefficient, time-consuming and expensive process.

Cambridge Healthtech Institute’s Seventh Annual Engineering Genes, Vectors, Constructs and Clones conference continues the tradition of applying effective engineering strategies for protein expression and production research leading to functional biotherapeutic products. Learn from seasoned, savvy researchers as they share their real-world experiences, applications and results.

Preliminary Agenda


Engineering Genes 

Gene and Protein Sequence Optimization for High-Level Production of Fully Active and A-Glycosylated Lysostaphin in Pichia pastoris 

Karl E. Griswold, Ph.D., Associate Professor, Bioengineering, Thayer School of Engineering, Dartmouth College  

The Engineering of Artificial Cellular Nanosystems Using Synthetic Biology Approaches 

Cheemeng Tan, Ph.D., Assistant Professor, Biomedical Engineering, University of California, Davis  

Enhance CHO Productivity Using RNAi Technology 

Jianxin Ye, Ph.D., Principal Scientist, Merck Research Labs  

Synthetic Biology: Bioengineering at a Systems Level 

Ilias Tagkopoulos, Ph.D., Assistant Professor, University of California, Davis   


Enabling Vectors 

Quantification of Cytosolic Plasmid DNA Degradation Using High-Throughput Sequencing: Implications for Gene Delivery 

Mark M. Banaszak Holl, Ph.D., Professor, Chemistry and Biomedical Engineering, University of Michigan  

Recombinant Protein Expression and Engineering: Generation of Expression Vectors and Protein Binders 

Agustín Correa, Ph.D., Research Scientist, Recombinant Protein Unit, Institut Pasteur de Montevideo  

Thinking beyond the Plasmid: Rapid Engineering of Stable Protein Expression and Improved Bacterial Strains Using Chromosomal Engineering   

Joseph D. Kittle, Jr., Ph.D., Assistant Professor, Chemistry and Biochemistry, Ohio University; Founder and CSO, MTL, LLC 


Enhancing Codons, Constructs and Clones 

A Scoring System for Predicting Solubility of Protein Constructs 

Brian Chiswell, Ph.D., Owner and Founder, Dimensions BioSciences  

A Condition-Specific Codon Optimization Approach for Improved Heterologous Gene Expression 

Hal Alper, Ph.D., Associate Professor, Chemical Engineering, University of Texas at Austin  

Use of CHO Cell Engineering for Improved Protein Secretion 

Pierre-Alain Girod, Ph.D., CSO, Selexis  

Production of Recombinant Protein by a Novel Oxygen-Induced System in Escherichia coli 

Joseph Shiloach, Ph.D., Head, Biotechnology Core Laboratory, National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health