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KEYNOTE PRESENTATION
qPCR: Historical Perspectives and New Publication Guidelines
Carl Wittwer, Ph.D., Professor, Pathology, University of Utah |
PCR’s sensitivity, specificity, and simplicity has revolutionized molecular biology in basic, industrial, and clinical settings. Technologies abound, including quantitative PCR (qPCR), multiplex qPCR, real-time PCR, and reverse transcription quantitative PCR (RT-qPCR). Your technology selection is determined by the source of nucleic acid and application. However, complexities arise because of multiple assays, numerous protocols, abundant sources, lack of assay standardization, differences in sample processing, ineffective use of controls, normalization methods, and quality control management. Researchers face challenges in reliability, relevance, and reproducibility. Learn from savvy, seasoned researchers as they share their real-world experiences, applications, and results.Comprehensive Coverage of:
- Pre-Analytical Sample Preparation: Extraction and Purification
- Novel Technical Approaches
- Data Models for Optimization
- Novel Applications
- Plus Sequencing Tech Expo
Pre-Conference Short Courses
Sunday, March 15 |
Getting the Most out of Your Real-Time PCR Experiments
Marwan Alsarraj, Product Manager, Gene Expression Division, Bio Rad Laboratories
How to do Successful Gene Expression Analysis Using Real-Time PCR
Jo Vandesompele, Ph.D., Professor, Center for Medical Genetics, Ghent University Hospital
Jan Hellemans, Ph.D., Center for Medical Genetics, Ghent University Hospital