AAV genomes don’t just stick around when capsids get thermally stressed – they leak out, and do it before proteins melt. However, methods that look only at protein unfolding don’t track DNA, so you’re not getting the whole story. This webinar will show how Uncle gives new insight on AAV stability by monitoring DNA ejection from capsids, in addition to protein unfolding and aggregation. The powerful ability to monitor protein and DNA behavior in one instrument gives a high resolution look into the ways AAV breaks down – and which capsids and conditions are the most stable. Beyond AAV stability, Uncle also packs tools to quantify encapsulated DNA and particle titer, all on the same small sample in under 2 hours.
- The two ways AAV genomes break out of their capsids and how to quantify escaped viral DNA
- How and when AAV genome release, aggregation, and protein unfolding occur
- Insights on stability and quality from measuring viral DNA before, during, and after a thermal ramp
Kevin Lance, Ph.D.
Kevin Lance is the Marketing Manager covering Uncle at Unchained Labs. He has published works and secured patents in the fields of sustained antibody delivery and nanotechnology and has worked as a consultant to advance the development of biologics for several major biopharmaceutical companies. He earned his Ph.D. in Bioengineering from the joint UC Berkeley – UCSF Bioengineering Graduate Program.
Cost: No Cost!