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Protein Production Lab Challenges: Methodologies, Strategies, and the Art of Managing Multiple Projects



Monday, January 25, 2021 | 1:00PM – 4:00PM(US ET)


Instructors:

Richard Altman, Field Application Scientist, Life Science Solutions, Thermo Fisher Scientific

Dominic Esposito, PhD, Director, Protein Expression Laboratory, Frederick National Laboratory for Cancer Research (FNLCR)


Workshop Description:
Protein expression laboratories provide crucial support to drug discovery efforts. As we would expect, there are numerous challenges in the effective operation of these critically needed facilities. This workshop will focus on the concepts, technologies, and strategies necessary to meet the ever-increasing need for recombinant proteins. This workshop combines instruction and case studies in an interactive environment.

Topics to be Covered:

  • How to Build an Effective Expression Facility
  • Basic Cloning, Expression, and Purification Systems
  • Prioritizing Projects or Asking the Right Questions
  • Total Workflow Efficiency
  • Engaging and Developing Team Members
  • The Importance of Tech Development to Long Term Success

Who Should Attend:

Protein Expression Scientists of all experience levels
Protein Core/Technologies Lab Managers/Members


Speaker Biographies:

Richard Altman, Field Application Scientist, Life Science Solutions, Thermo Fisher Scientific


Rich Altman has 30 years of experience in protein expression and production. In early 2019, he joined Thermo Fisher Scientific as a Field Application Scientist. Previously, he worked for several pharmaceutical companies, including Amgen, Alexion, Bayer, and Upjohn, on the cloning, expression, purification and characterization of recombinant proteins. This work supported both small-molecule high-throughput screening and protein therapeutic efforts. He received his MS degree from the University of Pittsburgh School of Medicine in the Department of Molecular Biology and Biochemistry.

Dominic Esposito, PhD, Director, Protein Expression Laboratory, Frederick National Laboratory for Cancer Research (FNLCR)


Dr. Esposito is currently the Director of the Protein Expression Laboratory (PEL) at the Frederick National Laboratory for Cancer Research. The PEL focuses on production of high-quality Ras pathway-related proteins for the National Cancer Institute’s RAS Initiative. These proteins are being used for a wide-ranging attack on Ras biology including structural biology, biophysics, and development of screens and assays for drug discovery. Dr. Esposito received his B.A. in Chemistry at La Salle University in Philadelphia, and his Ph.D. in Biochemistry at the Johns Hopkins University Bloomberg School. Dr. Esposito previously worked for Life Technologies, where he helped to develop the Gateway recombinational cloning system.