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Advances in High-Throughput Screening of Aggregation, Stability and Viscosity Utilizing Light Scattering in Microwell Plates




October 26, 2017
11 am to 12 pm EDT

 

Sponsored by
Wyatt Tech

Preview:

 

Webinar Description:

Aggregation and stability are important criteria for selecting biotherapeutic candidates for developability and optimizing their formulation. Screening of the broadest matrix of conditions and excipients enables true QbD, imparting confidence in the ultimate choice of candidates and formulations with which to move forward.

The primary biophysical techniques for characterizing aggregation and stability in a high-throughput, broad-matrix setting are based on light scattering. Dynamic light scattering (DLS) determines size and size distributions, while changes in apparent size and aggregation with temperature (Tm, Tagg) and concentration (kD) are indicative of conformational stability and colloidal stability, which in turn help predict propensity for aggregation. Turbidity and aggregation estimates are also obtained from the static light scattering information inherent in DLS measurements.;

DLS has been available for over a decade in a plate reader format for high—throughput screening of size, aggregation, Tm, Tagg and kD. The DLS plate reader also serves to measure the viscosity of concentrated protein solutions. This webinar introduces the next generation DLS plate reader, which adds new capabilities for extended characterization of the biophysical properties and stability of biotherapeutics.


Speakers:

Daniel SomeDaniel Some, Ph.D.

Principal Scientist

Wyatt Technology Corp.


Daniel Some is Principal Scientist at Wyatt Technology Corp. He has been with Wyatt for over twelve years, first in R&D and then in the marketing department. Prior to joining the scientific instrumentation world his professional endeavors included the semiconductor and defense industries. Dr. Some completed his undergraduate degree in physics at the Technion Israel Institute of technology, his doctoral research in the Brown University physics department, and postdoctoral research at Los Alamos National Lab and the Weizmann Institute of Science.



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