Optimizing Protein Biotherapeutic Formulations with the Light Scattering Toolkit



April 20, 2016

 

Sponsored by
Wyatt Tech

Webinar Description:

Biophysical techniques based on static and dynamic light scattering address many of the key analytical challenges in biotherapeutic formulation development, including screening for aggregation, colloidal and conformational stability, viscosity and chemical modifications. This seminar will review light scattering technology and instrumentation, then present select examples illustrating how complete light scattering solutions facilitate rapid and effective development of protein-based biotherapeutic formulations.

Learning Objective Bullet Points:

  • The light scattering technologies and instrumentation relevant to protein biotherapeutic formulation
  • How a dynamic light scattering (DLS) plate reader is used to pre-screen dozens of formulations for aggregation, propensity for aggregation and viscosity
  • How multi-angle light scattering couples to HPLC- or UHPLC-based size-exclusion chromatography (SEC-MALS) and field-flow fractionation (FFF-MALS) to characterize soluble and insoluble aggregates, as well as fragments and other degradation products
  • How composition-gradient multi-angle light scattering (CG-MALS) characterizes reversible self-association and co-solute interactions
  • How automated electrophoretic light scattering (MP-PALS) characterizes pI and zeta potential of proteins, directly in formulation buffer.

Speaker Info:

Daniel_SomeDaniel Some, Ph.D.

Principle Scientist and Director of Marketing

Wyatt Technology Corp.


As Director of Marketing, Dr. Some is responsible for conveying to customers the manifold benefits of our unique products and services, as well as learning about specific needs and opportunities expressed by our customers. He believes that our success can only be achieved through that of our users. As Principal Scientist, he investigates techniques for characterization of macromolecular interactions, contributing to the development of new instruments and applications. Dr. Some obtained his B.Sc. from the Israel Institute of Technology and Ph.D. from Brown University, both in Physics, and completed his postdoctoral research at Los Alamos National Laboratory and the Weizmann Institute of Science. Prior to joining Wyatt Technology, he worked on R&D of semiconductor wafer inspection tools and defense electro-optics.



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