Upcoming Global Web Symposia
December 8, 201112:30 pm – 2:00 pm EST
Symposium Course Description: The origins of the yeast Pichia pastoris will be discussed along with the logic and research that lead to its eventual development as a host for the production of recombinant proteins. Recent advances in vectors and host strains for its use will be presented. An example of its use, the cloning and expression of a full-length chimeric humanized monoclonal antibody, will be presented.
Learning Objective Bullet Points:
Who Should Attend: Researchers of all levels from B.A. to Ph.D. interested in synthesizing recombinant proteins.
Instructor Information:James M. Cregg, Ph.D. Professor, Keck Graduate Institute of Applied Life Sciences, Claremont, CA; Principal Scientist and Chairman of the Board, Biogrammatics, Inc., Carlsbad, CA.
Ph.D. degree, Rice University, Houston, TX, 1978; Postdoctoral fellow, Scripps Clinic and Research Foundation, La Jolla, CA, 1978-1980; Staff Scientist, BRL, Inc., 1980-1982; Senior Staff Scientist, SIBIA, La Jolla, CA, 1983-1989; Associate Professor, Oregon Graduate Institute, Beaverton, OR, 1989-1999; Professor, Keck Graduate Institute, Claremont, CA, 1999-present; Executive editorial board for journal Protein Expression and Purification, 1999-present ; Chairman of the Board, Biogrammatics, Inc., Carlsbad, CA, 2008-present; Principal Scientist, Biogrammatics, Inc., Carlsbad, CA, 2011-present.
Recent Publications: 1. Cregg, J. M., Tolstorukov, I., Kusari, A., Sunga, J., Madden, K., and Chappell, T. (2009) Expression in the yeast Pichia pastoris. Methods Enzymol.;463:169-89.2. Zhang, P., Zhang, W., Zhou, X., Bai, P., Cregg, J. M.2, and Zhang, Y. (2010) Role of two Pichia pastoris hexose transporter homologues in sugar uptake and catabolite derepression (sugar-induction) of AOX1 promotor. Appl. Environ. Microbiol. 76:6108-6118.3. Ternes, P., Wobbe, T., Schwarz, M., Albrecht, S., Feussner, K., Riezman, I., Cregg, J. M., Heinz, E., Riezman, H., Feussner, I., and Warnecke, D . (2011) Two pathways of sphingolipid biosynthesis are separated in the yeast Pichia pastoris. (in press).4. Nazarko, V. Y., Nazarko, T. Y., Farre, J. C., Stasyk, O. V., Warnecke, D., Ulaszewski, S., Cregg, J. M., Sibirny, A. A., and Subramani, S. (2011) Atg35, a micropexophagy-specific protein that regulates micropexophagic apparatus formation in Pichia pastoris. Autophagy 7:375-385.5. Jeffries, TW and Cregg, JM. (2010) Protein expression in non-conventional yeasts. Manual of Industrial Microbiology, 3rd ed. American Society for Microbiology R. H. Baltz, J. E. Davies and A. L. Demain, Editors. ASM Press, Washington DC. pp. 302-317.6. Cregg, J. M., Tolstorukov, I., Kusari, A., Sunga, A. J., Madden, K., Chappell, T. (2011) Expression of recombinant genes in the yeast Pichia pastoris. Curr. Protoc. Essen. Lab. Tech. 13.2.2-13.2.14 (in press).
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