2014 Archived Content
MONDAY, OCTOBER 6
9:00 am - 12:00 pm
Digital PCR Experiment Design and Primer
Instructors: Jim Huggett, B.Sc. (Hons), Ph.D., Science Leader, Nucleic Acid Metrology, Molecular & Cell Biology, LGC
Yann Jouvenot, Ph.D., Product Manager, Digital Biology Center, Bio-Rad Laboratories
This course will introduce the concept of digital PCR (dPCR) and explain how it compares to other molecular methodologies, citing both advantages and disadvantages. Included in the discussion will be the application of dPCR to perform minority target detection, absolute quantification and measurement of copy number variation as well as the analysis of RNA using reverse transcriptase dPCR. The discussion will also include some of the specific challenges associated with performing this technique.
TUESDAY, OCTOBER 7
6:00 - 8:00 Dinner Short Course: Single-Cell Genomic Analysis*
Daniel T. Chiu, Ph.D., A. Bruce Montgomery Professor, Chemistry; Endowed Professor, Analytical Chemistry; Professor, Bioengineering, University of Washington, Seattle
Jason Kreutz, Ph.D., Senior Scientist, Chemistry, University of Washington, Seattle
Amy Paguirigan, Ph.D., Staff Scientist, Fred Hutchinson Cancer Research Center
* Separate registration required