SUNDAY, JUNE 7
11:30 - 12:00 Short Course Registration
12:00-3:00 pm
The course is designed to provide in-depth information on how to go about setting up RNAi screening experiments and how to design assays for getting optimal results. The challenges working with different types of molecules i.e. siRNAs, shRNAs and the delivery systems to get them into the appropriate cells and tissues will be discussed. The instructors will also provide their input on best practices for the execution of experiments and interpretation of results when dealing with complex biology and informatics.
Topics to be covered:
Overview of assay designs for siRNA and shRNA screens
Testing and validating methods for RNA delivery
Use of appropriate reagents and cell types
Implementing proper quality control
Minimizing cytotoxicity and off-target effects
Data analysis and statistical considerations
Instructors:
Christophe J. Echeverri, Ph. D., CEO/CSO, Cenix BioScience GmbH
Marc Ferrer, Ph.D., Senior Research Fellow, Automated Biotechnology, Merck & Co.
3:30-6:30 pm
The course is designed to provide both the beginner and the expert, with a comprehensive overview of current and emerging delivery systems and formulations facilitating RNAi delivery. Various methods for RNAi delivery such as viral vectors, liposomes, nanoparticles, chemical conjugates and other commonly used techniques will be compared and contrasted for different applications. The instructors will also discuss the challenges associated with RNAi delivery, both in vitro and in vivo, and offer guidance based on their knowledge and expertise in the field.
Topics to be covered:
Testing and validating methods for delivery
Overview of viral and non-viral vector systems
Chemical methods of improving delivery
New formulations to optimize delivery
Minimizing cytotoxicity and off-target effects
Examining tissue distribution and safety profiles
Instructors:
Muthiah Manoharan Ph.D., Vice President, Drug Discovery, Alnylam Pharmaceuticals
Roger Adami, Ph.D., Associate Director, Molecular Pharmaceutics, MDRNA Inc.
John Rossi, Ph.D., Professor and Chair, Molecular Biology, Beckman Research Institute of the City of Hope
Namjin Chung, Ph.D., Senior Research Investigator, Applied
Genomics, Bristol-Myers Squibb
For more conference information, please contact:
Tanuja Koppal, Ph.D.
Conference Director
Cambridge Healthtech Institute
Phone: 973-525-4667
Email:
tkoppal@healthtech.comFor partnering and sponsorship information, please contact:
Jon Stroup
Manager, Business Development
Cambridge Healthtech Institute
Phone: 781-972-5483
Email: jstroup@healthtech.com