Day 1 | Day 2
Thursday, August 27, 2009
7:30am Morning Coffee (Sponsorship Opportunity Available – Please contact Suzanne Carroll at scarroll@healthtech.com)
8:25 Chairperson’s Remarks
Xiaotian Zhong, Ph.D., Principal Scientist & Lab Head, Mammalian Expression, Wyeth Research Labs
8:30 Heparin and Unfolded Protein Response Factors for Protein Expression Optimization in Mammalian Cells
Xiaotian Zhong, Ph.D., Principal Scientist & Lab Head, Mammalian Expression, Wyeth Research Labs
Cultivated mammalian cells, such as CHO and HEK293, produce active recombinant target proteins with relevant post-translational modifications. The main challenges remain how to optimize protein expression and therefore improve protein yields. The presentation will take the opportunity to summarize our recent advances with expression optimization by using heparin and protein factors involved in Unfolded Protein Response pathways. Case studies on the expression optimization of Secreted Fizzled-Related Protein-1 and Low-Density Lipoprotein Receptor-like family will be reported. The molecular mechanisms of the applications and the discoveries will also be discussed.
9:00 Metabolic Characterization of Mammalian Cell Lines using Quantitative Targeted Metabolomics
Denise Sonntag, Ph.D., Senior Scientist Biochemistry, BIOCRATES Life Sciences AG
Information about the metabolic capability of mammalian cell lines is crucial for the selection of productive clones. It could be demonstrated that a quantitative targeted metabolomics approach is suited to facilitate the selection process, as it enabled a fast and comprehensive characterization of cell line-specific metabolism. Small sample amounts of cell culture supernatants or cell lysates were sufficient to simultaneously quantify several hundred metabolites of different classes, e.g. amino acids, intermediates of the energy metabolism, phospholipids, sugars, and biogenic amines, using sensitive and specific mass spectrometry methods (API-MS/MS). Examples of application will be presented to highlight the potential of targeted metabolomics for mammalian cell line characterization and optimization.
9:30 Bypassing Conventional Approaches for Rapid Discovery and Optimization of Rare-Event Cell Lines Using ClonePix FL
Christopher Mann, Ph.D., Senior Applications Support Specialist, Applications Support Team, Genetix
A major bottleneck in both discovery and development of cell lines is finding and isolating the best cell clones from large heterogeneous cell populations. High-throughput automation is one option to handle the workload but a better solution is to rapidly find and isolate only the best candiDate, thereby failing thousands of irrelevant clones early. Latest applications and case studies will be cited for 1) finding antigen-specific clones from hybridoma fusions, 2) isolating the highest producers from transfections, and 3) early detection and elimination of clone instability by measuring heterogeneity of antibody secretion from daughter clones.
10:00 Networking Coffee Break with Exhibit and Poster Viewing
10:30 Automated Selection and High-Throughput Genomic and Epigenomic Profiling of Cell Lines Expressing Secreted and Non-Secreted Recombinant Proteins
Zulkeflie Zamrod, Ph.D., Vice President, Research & Development, Inno Biologics Sdn Bhd
We have recently used an automated method employing ClonePix FL instrument to select high producers expressing a humanized antibody (secreted protein) and reporter protein (non secreted). Time and labor required to obtain stable cell lines were reduced by at least 50% by using this automated method. Gene expression microarray and ChIP-Seq were used to obtain the genomic and epigenomic profiles of high producers compared with the low ones. Transgene integration sites were also characterized. Existing as well as newly developed statistical and computational methods were used to analyze and predict common and disparate molecular regulatory events of secreted and non secreted protein expression.
11:00 Automating Cell Line Development Using the CELLO Robotic System
Ulrica Skoging-Nyberg, Ph.D., Team Manager, Recipharm Biologics
11:30 Label-Free Separation of Cells by Rolling on Patterned Receptors
Rohit Karnik, Ph.D., Assistant Professor, Mechanical Engineering, Massachusetts Institute of Technology
Rapid separation of cells with minimal modification is highly desirable for retaining cell viability and phenotype for applications in therapeutics and diagnostics. Current techniques for cell separation often involve labeling, washing, or other invasive steps that have the potential to alter the cell phenotype. We are exploring a new biomimetic method to separate cells based on cell rolling, which is a physiological phenomenon that plays a role in recruitment of cells from the blood stream. Cell rolling involves transient formation and dissociation of bonds between the cell and the endothelium, and is exhibited by mammalian cells including leukocytes, stem cells, and some cancer cells. We have discovered that combining cell rolling with asymmetric receptor patterning enables control of the trajectories of the rolling cells in a microfluidic device. It opens the possibility for label-free cell separation where a stream of cells could be separated and collected in a continuous-flow manner in a simple device with minimal to no sample processing. In this talk, I will present our work on controlling cell rolling and developing a microfluidic device for separation of cells. Future development of this technology may lead to simple, label-free methods for separation of stem cells, cancer cells, neutrophils, and other blood cells for therapeutic and diagnostic applications.
12:00pm End of The Bioprocessing Summit
For more information, please contact:
Mary Ruberry, Conference Director
Cambridge Healthtech Institute
Phone: 781-972-5421
E-mail:
mruberry@healthtech.comFor sponsorship information, please contact:
Suzanne Carroll, Manager, Business Development
Cambridge Healthtech Institute
Phone: 781-972- 5452
E-mail: scarroll@healthtech.com