Monday, August 24, 2009
8:00am Pre-Conference Registration & Morning Coffee
Pre-Conference Short Course*
(SC1) OPTIMIZING MEDIA: ACHIEVING SUPER SOUP
9:00am-12:00pm
Experts agree that optimizing media is a key component for increasing cell density. This workshop covers the details necessary for achieving Super Soup, from oxygen uptake to nutrient feed and beyond. Join your colleagues and media experts as they reveal solutions for enhancing cell culture media in the quest to achieve ever higher yields.
9:00am Design and Optimization of the Cell Culture Medium
Paul J. Price, Ph.D., Chief Scientific Officer, D Finitive Cell Technologies Inc.
The cell culture medium is a dynamic mixture consisting of amino acids, vitamins, a source of energy, growth factors, trace minerals and other components in a buffered salt solution. Each component has a shelf life, sensitivity to the physical environment and a variety of interactive and break-down products. The Classical mammalian cell culture formulations require further supplementation with a protein source such as serum and were designed using cancer-derived cell lines. Serum supplemented formulations for mammalian cells can be very sub-optimal for the growth of cells at high cell concentrations and for the isolation of recombinant products, antibodies or virus. This seminar will start with the role and problems associated with some of the key basic components of the medium and then show how Industry has been able to progress from serum-requiring to serum-free, to animal-component-free and then to chemically-defined formulations. Emphasis will be placed on how the cell culturist can reduce apoptosis by controlling the physical environment and the production of ammonia and free radicals through the optimization of the media formulation and the proper handling of the cells.
9:45 Rationale for Monitoring Metabolic Parameters in Cell Culture Processes
Timothy Fawcett, Ph.D., Director, The BioTechnical Institute of Maryland, Inc.
Cell culturing conditions play an important role in providing a suitable environment for the growth and development of animal cells. As expectations for productivity and reproducibility increase, a closer examination of mammalian cell culture media components is necessary. Therefore, we will discuss key media components, their reason for inclusion in media and the positive and negative results of decay, and metabolic breakdown. A rationale for monitoring some key media constituents and metabolites will also be given along with suggestions to enhance culturing conditions and increase productivity.
10:30 Coffee Break
11:00 Microengineering Approaches for Controlling the Cellular Environment and Media Optimization
Ali Khademhosseini, Ph.D., Professor, Harvard-MIT Division of Health Sciences and Technology, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School
In this talk I will discuss our group's work in using microtechnologies such as microfluidics, micropatterning and micromolding of biomaterials for controlling the cellular microenvironment for regulating stem cell behavior and tissue formation.
11:45 Interactive Discussion with Speakers
12:00pm Close of "Optimizing Media" Short Course
Instructor Bio Sketches:
Dr. Timothy Fawcett
Dr. Fawcett earned his undergraduate degree in Biochemistry from the University of New Hampshire, and his doctorate in Biochemistry from Louisiana State University. Prior to joining BTI in May 2001, he was Training Center Manager of Life Technologies, Inc. / Invitrogen. There he wrote, developed, and presented workshops designed to teach medical doctors, principal investigators, and laboratory technicians in areas such as cell culture, recombinant DNA, and protein expression through a combination of lecture and laboratory exercises. Dr. Fawcett has investigated molecular responses to cellular stress at the National Institute on Aging, and the National Institutes of Health in the Laboratory on Gene Expression and Aging. In addition, he consults in the industry. As Director of The BioTechnical Institute of Maryland, Dr. Fawcett leads BTI's Workshop Program and assists with curriculum development.
Dr. Ali Khademhosseini
Ali Khademhosseini is an Assistant Professor of Medicine and Health Sciences and Technology at Harvard-MIT's Division of Health Sciences and Technology and the Harvard Medical School. His research is based on developing micro- and nanoscale technologies to control cellular behavior with particular emphasis in developing microscale biomaterials and engineering systems for tissue engineering and drug delivery. He has published 2 edited books, 80+ peer reviewed papers, 20 book chapters, 100+ abstracts, and 17 issued or pending patents. He has also been invited to give over 100 seminars world-wide. His accomplishments have been recognized by a number of awards including the Outstanding Undergraduate Research (UROP) mentor at MIT (2004), the Outstanding Researcher in Polymer Science by OMNOVA / MIT (2005) and the Coulter Foundation Early Career Award (2006). Also, he won the BMW Scientific Award (2007), one of the most prestigious international young innovator awards and the ACS Victor K. LaMer award (2007) for his doctoral thesis. In 2007, he was recognized as one of the top young innovators (TR35) by the Technology Review Magazine. He has also been the recipient of the NSF CAREER award (2009), as well as the Early Career awards of the University of Toronto’s Engineering School - class of 7T6 (2009) and the IEEE-EMBS society (2008), which is the largest international organization for biomedical engineers. He received his Ph.D. (2005) in bioengineering from MIT under the supervision of Prof. Robert Langer, and MASc (2001) and BSc (1999) in chemical and biomedical engineering from the University of Toronto.
Dr. Paul J. Price
I have been active in the areas of cell culture and media development since 1958. I received my PhD from the George Washington University with majors in Cell Biology, Biochemistry and Virology, and I am currently the Chief Scientific Officer of D-Finitive Cell Technologies (DCT), a specialty cell media company. I am also on the Scientific Advisory Board and a consultant to Sigma-Aldrich, Regenerative Medicine and Research Cell Culture and an Adjunct Professor at the Medical University of South Carolina, Department of Regenerative Medicine. Previously I was a Branch Chief in the Center for Infectious Diseases at the CDC and later founder and Executive Vice-President of Hycor Biomedical (later called Stratagene). As part of R&D at GIBCO Invitrogen/Life Technologies, I helped design and develop much of their specialized media for the culture of neurons, stem cells and other mammalian cells, including those used in Bio-Production. As CSO of DCT, I have developed numerous animal-origin-free formulations for many of the same cell types. I have been an active member of the Society for In Vitro Biology since 1959 and a member of their Board since 1998 and Society President from 2006-2008. I have been a reviewer for “In Vitro” and other journals, and an outside expert for grant reviews for the NCI.