Recorded October 21, 2015

Sponsored by
NanoTemper Technologies logo

Webinar Description:

The course will introduce the Prometheus NT.48 instrument (NanoTemper Technologies) and its novel nanoDSF technology, which allows for parallel high-precision characterization of stability and aggregation parameters of biologicals. A large number of samples can be analyzed for their thermal and chemical stability as well as aggregation properties in a volume of 10 µl, and in a concentration range from just 5 µg/ml up to > 200 mg/ml. This way, information-rich stability data can be generated rapidly to guide processes in formulation development, protein engineering, assay development, drug discovery or quality control.

Learning Objectives:

  • Introduction to nanoDSF technology: Measurement principle for fluorescence and aggregation detection.
  • Handling: How to prepare samples and how to use the software to set up experiments on the Prometheus NT.48.
  • Case Studies:
    • Simultaneous fluorescence and aggregation detection for in-depth biological characterization.
    • nanoDSF for formulation development and antibody engineering.
    • Characterization of membrane protein stability by detergent screens.


Dennis_BreitsprecherDr. Dennis Breitsprecher

Head of R&D Biochemistry

NanoTemper Technologies


Dennis Breitsprecher studied Biochemistry at the University of Hannover, Germany, with a focus on Biophysical Chemistry. For his PhD work, he joined the Institute of Biophysical Chemistry at Hannover Medical School, and graduated in 2010 with Magna Cum Laude. He then moved to Boston, MA, where he conducted a project funded by the German research foundation to establish real-time single molecule imaging to characterize multi-protein complex interactions at Brandeis University. He is the author of >20 scientific publications and won the young scientist award of the German Society for Cell Biology in 2013. He then joined NanoTemper Technologies in Mai 2013 to use his expertise in protein biochemistry to develop solutions for life-science research.

Since 2013 Research & Development at NanoTemper Technologies.
2010-2013: Post-doc at Brandeis University, Waltham, MA, funded by the German research foundation.
2010: PhD in Biochemistry, Hannover Medical School, Germany.


Stefan_DuhrDr. Stefan Duhr


NanoTemper Technologies


Stefan Duhr studied Biochemistry at the University of Witten/Herdecke, Germany with a specialization in Physical-Biochemistry. In 2004, he began his PhD studies in the Physics department at the Ludwig-Maximilians-University Munich. He finished his work with the title "Thermo-Optical Molecule Manipulation" in the group of Prof. Dieter Braun in June 2007 with Summa Cum Laude. In May 2008, he founded NanoTemper Technolgies GmbH together with Dr. Philipp Baaske. Stefan Duhr is Geschaeftsfuehrer (CEO) of NanoTemper Technologies, which was since then awarded with multiple prices, including the German Innovation Award in 2012 and the German Founders Award in 2014.

2008: Co-founder of NanoTemper Technologies.
2004-2007: PhD in Physics, LMU Munich, Germany.
2000-2004: MSc in Biochemistry, University of Witten/Herdecke, Germany