March 11, 2021
11 am to 12 pm EST
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Simultaneous, High-Yield Extraction of DNA and RNA from FFPE Tissue – Purigen Biosystems Ionic®

Webinar Description:

FFPE tissue and samples with a low abundance of high-quality nucleic acid represent a bottleneck in sample preparation workflows. Researchers and clinicians working with these samples often resort to labor-intensive and potentially biased methods in order to isolate sufficient material for downstream analyses.

Purigen Biosystems has recently launched the revolutionary Ionic® Purification System and Kits to recover more RNA and DNA from FFPE samples with a simple, automated approach using isotachophoresis. The new Ionic® FFPE Complete Purification Kit combines the benefits of the RNA and DNA extraction kits for FFPE samples into a single workflow with minimal hands-on time.

Purigen’s core technology, isotachophoresis, separates and concentrates charged molecules in solution solely based on their electrophoretic mobility. Biological samples are gently lysed and added to the Purigen Ionic® Fluidic Chip. An electric field is then applied to the chip and the nucleic acid is isolated in its natural, native form. The nucleic acid is not denatured or dehydrated, and there’s no binding to, or stripping from, fixed surfaces.The result is a higher yield of pure nucleic acid that is less fragmented and free from bead or wash buffer contamination.

In this webinar, we share results from downstream molecular analyses of RNA and DNA purified from FFPE samples in a single workflow using the Ionic® Purification System.

Learning Objectives:

Attendees will learn about Purigen Biosystems’ innovative approach to automated nucleic acid purification and how the Ionic® Purification System can be used to extract more DNA and RNA from challenging FFPE samples with more reliability and less effort by comparison to conventional extraction methods.

Speaker:

Doug Fadrosh
Senior Scientist
Purigen Biosystems

Doug joined Purigen Biosystems in September 2019 as a Senior Scientist to support ongoing development programs and lead Purigen’s proof-of-performance program. Doug previously managed the Microbiome Sequencing Core at UCSF and was a key member of a multi-institutional collaborative project to investigate the role of the microbiome in early onset childhood asthma. He was previously a Laboratory Manager and Senior Bioinformatics Analyst at the Institute for Genome Sciences and a Quality Control Manager at Cosmos ID in addition to holding Research Associate positions at The J. Craig Venter Institute and The Institute for Genomic Research. Doug received a master’s degree in Biotechnology at The John Hopkins University.


Cost: No Cost!