Webinar Description:

Given the complexity of biological macromolecules used for therapeutic purposes, detailed characterization is both essential and challenging. Some of the most important tools for analyzing the properties and behavior of biologics are based on multi-angle light scattering and dynamic light scattering, which directly determine molar mass, size, stability and interactions. This webinar will provide a brief introduction to these techniques, then several case studies will be presented that utilized dynamic light scattering (DLS), multi-angle light scattering with size-exclusion chromatography (SEC-MALS), and composition-gradient multi-angle light scattering (CG-MALS) as analytical tools for the characterization and formulation of macromolecules ranging from virus-like particle-based vaccines to monoclonal antibodies.

Key Learning Objectives:

• The technology and capabilities of DLS, SEC-MALS and CG-MALS
• How DLS is applied to study the aggregation and, stability of biologics
• The importance of SEC-MALS for determining absolute molar mass and size of proteins, complexes and self-assemblies
• How composition-gradient light scattering characterizes macromolecular interactions to better understand formulation stability and propensity to aggregate

Speakers:

Ozan Kumru, PhD
Research Assistant Director, Vaccine Analytics and Formulation Center
University of Kansas, Lawrence, KS

Ozan Kumru received his B.S. and Ph.D. in Microbiology from Kansas State University and the University of Kansas School of Medicine, respectively. For the past 10 years, he has worked in the Vaccine Analytics and Formulation Center within the department of Pharmaceutical Chemistry at the University of Kansas. Under the leadership of Dr. David Volkin and Dr. Sangeeta Joshi he has performed analytical characterization, analytical method development, pre-formulation and formulation of a diverse set of macromolecules. Representative examples include peptide-based conjugate drugs, monoclonal antibodies, live-attenuated viral vaccines and immunotherapy vectors.

Dr. Kumru has experience in a wide range of analytical instrumentation and including light scattering (DLS, SLS, SEC/CG-MALS), analytical ultracentrifugation, calorimetry, chromatography, spectroscopy, immunoassays, and cell-based virus titration assays. He has been involved in characterization and formulation of several drugs and vaccine candidates that are licensed products or are currently undergoing evaluation in clinical trials.

Dan Some, Ph.D.
Principal Scientist
Wyatt Technology

Dr. Dan Some is Principal Scientist at Wyatt Technology Corp. where he contributes to product and application development as well as scientific and technical marketing. He was instrumental in leading Wyatt’s program for instrumentation to characterize biomolecular interactions and has also made significant contributions to the real-time process analytics technology effort. Dan studied undergraduate physics at the Technion—Israel Institute of Technology and obtained his Ph.D. in physics from Brown University, then carried out postdoctoral research at Los Alamos National Laboratory and the Weizmann Institute of Science. Prior to joining Wyatt he was involved in defense technology and the development of processed wafer inspection tools for the semiconductor industry.

Cost: No Cost!