SUNDAY, APRIL 20
1:30 Short Course Registration
2:00-5:00 Introducing PREXCEL-Q: An Excel-based Software Program for the Rapid Design of Fail-Safe, Dynamically-Sound One- and Two Step qPCR Reactions
Jack M. Gallup, Assistant Scientist III, Department of Veterinary Pathology, Iowa State University
BRIEF COURSE DESCRIPTION:
Mr. Jack Gallup will describe the theory behind PREXCEL-Q and then guide participants through the use of the program for one-step qPCR, two-step qPCR and LCM-qPCR set-ups using real-life examples in each case. Attendees will learn the automatic command functions of the program which accomplish thousands of crucial qPCR set-up calculations in minutes instead of hours or days, etc.
WHO SHOULD ATTEND?
Any researcher who is responsible for setting up qPCR reactions in the lab who knows first-hand the tedium and frustration often associated with the responsible execution of qPCR set-ups. Those who wish to save time while designing reliable qPCR reactions.
WHY ATTEND?
Even though PREXCEL-Q is new, it is already proving to be an invaluable tool to qPCR users across the globe. It automatically calculates all sample dilutions, standard dilutions, master mix set-ups for five major company’s most popular Master Mixes (for Test Plate [‘validation plate’], final qPCR plates and NRC [or NAC] plates), DNase and RNase reaction formulations, RT reaction assemblies, etc. It is a truly helpful and welcome implement, and can be licensed for free use by all academic institutions.
Hosted by
2:00-5:00 Getting the Most out of Your qPCR Experiments
Agenda:
- Review of critical assay design parameters
- Optimization of assay performance
- Troubleshooting:
- Contamination
- Primer Dimers
- Efficiency/Reproducibility/Sensitivity
- Analysis Considerations:
This will be an interactive workshop - attendees are encouraged to bring data files and problem examples with them.
Who Should Attend & Why
All levels of qPCR users are encouraged to participate, you will learn good laboratory practices to help you optimize your qPCR assays and effectively troubleshoot your results.
* Separate Registration Required
4:30-5:30 Early Conference Registration