WEDNESDAY, APRIL 23
TECH EXPO • 8:30 am - 12 pm
7:30-8:15am Conference Registration and Morning Coffee
TECH EXPO
Explore available next-generation screening platforms as presented by sequencing leaders. An unparalleled opportunity to compare and contrast these next-generation sequencing platforms to best suit your research needs.
8:15 Chairperson’s Opening Remarks
Sponsored Seminars hosted by:
| 8:30 True Single Molecule Sequencing: Current Research and Our Path to the $1000 Genome |
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Patrice M. Milos, Ph.D., Vice President and Chief Scientific Officer, Helicos
Helicos has developed a novel genetic analysis platform to efficiently and accurately determine the direct sequence of individual DNA molecules. Simplicity in sample preparation, development of novel surfaces, chemistry to enable incorporation of single nucleotides into DNA strands and finally the visualization of fluorophore addition to monitor real-time sequencing by synthesis has been achieved. This platform will provide the opportunity for researchers to interrogate the genome on a new scale and provides a path to sequence individual genomes at a cost to make integration of genome knowledge and healthcare possible. To demonstrate the current power of the HelicosTM platform we will discuss the use of the True Single Molecule Sequencing Technology (tSMSTM) for candidate gene resequencing, RNA measurements and miRNA analyses. |
| 9:15 Illuminating the Genome |
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Abizar Lakdawalla, Ph.D., Senior Product Manager, Sequencing Applications, Illumina
The Illumina Genome Analyzer, based on the Solexa massively parallel sequencing-by-synthesis technology, is being used for a broad set of functional genomics applications including chromosomal re-arrangements, to single nucleotide variations, variation in DNA methylation, whole transcriptome analysis, small RNA analysis, digital gene expression, DNA-protein, and DNA-RNA interaction analysis. Details on the current state of the technology as well as a summary of chromosomal resequencing studies, whole genome epigenetic changes, tissue-specific mRNA splice variatns and 5’-UTRs, microRNAs and DNA-protein interactions studies will be presented. |
10:00 Making High Throughput Whole Genome Sequencing Routine
Todd Arnold, Ph. D., Director of Molecular Biology, Roche 454 Life Sciences
As demonstrated by over 140 peer reviewed publications, researchers have successfully Roche/454 Genome Sequencer FLX platform for exploration and elucidation of many genome-related questions. Recent results from viral metagenomics, whole genome sequencing of complex organisms, and various transcriptome studies will be presented. The GS FLX currently provides read lengths of 250 base pairs generating 400,000 reads per 7.5 hour instrument run. Advances in the system will be presented, including data demonstrating read lengths in excess of 400 base pairs, over 1 million reads per instrument run, and improved data quality. These system advancements are achieved solely through reagent and software updates and do not require changes to the current GS FLX system hardware.
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10:45 Networking Coffee Break, Poster and Exhibit Viewing
11:30 Interactive Panel Discussion: The $1000 Genome Threshold
Moderator: Kevin Davies, Ph.D., Editor-in-Chief, BioIT World
The past 30 months have witnessed remarkable advances in next-generation sequencing throughput, accuracy, and results. More than 200 instruments have now been deployed in organizations around the world, and the range of questions and applications being addressed continues to astound. Together with rapid advances in consumer genomics, we are fast approaching the Holy Grail of the $1000 human genome sequence. This panel discussion will review the most recent technical advances and scientific applications of next-generation sequencing, as well as consider the practical, medical and ethical issues surrounding personalized genomics.
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